Studies using HPLC-PDA in gabapentin N-derivatization reactions with 9-fluorenylmethyl chloroformate (FMOC-Cl)
| Autor: | Rosane Nora Castro, Marco Edilson Freire de Lima, Heloisa Justen Moreira de Souza, Carla Regina Gomes Rodrigues Santos, Emanoel Hottes |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Química Nova v.44 n.5 2021 Química Nova Sociedade Brasileira de Química (SBQ) instacron:SBQ Química Nova, Volume: 44, Issue: 5, Pages: 543-546, Published: 26 JUL 2021 Química Nova, Vol 44, Iss 5, Pp 543-546 (2021) |
| Popis: | FMOC-Cl is a derivatizing agent widely used both in N-protection reactions of a-amino acids as well as in quantification and detection of amino acids and related molecules using HPLC-PDA or fluorescence analysis. In this work we studied the reaction of FMOC-Cl with gabapentin. However, it was observed that the degradation of gabapentin-N-FMOC and FMOC-Cl to FMOC-OH occur simultaneously in presence of the basic reaction medium which is specific for gabapentin derivatization. The degradation reaction is accelerated in temperatures above 45 °C and also by the time of contact of the reactant and product with basic medium. In this study, gabapentin derivatization reactions were shown to be cleaner and more efficient when an FMOC-Cl/gabapentin ratio of 2:1 was used, at 25 °C over a period of 15 minutes. |
| Databáze: | OpenAIRE |
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