Ferrerol overcomes the invasiveness of lung squamous cell carcinoma cells by regulating the expression of inducers of Epithelial Mesenchymal Transition
| Autor: | Jinlan Gong, Hui Yao, Bin Li, Peng Chen, Li Li, Jie-Hua Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition Lung Neoplasms Cell Survival Cell Vimentin Biology Microbiology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Antigens CD Cell Movement Cell Line Tumor medicine Humans MTT assay Neoplasm Invasiveness Epithelial–mesenchymal transition Cell Proliferation Wound Healing Mesenchymal stem cell Zinc Finger E-box-Binding Homeobox 1 Cadherins Blot 030104 developmental biology Infectious Diseases medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Cancer research biology.protein Carcinoma Squamous Cell Snail Family Transcription Factors Wound healing Drugs Chinese Herbal Signal Transduction |
| Zdroj: | Microbial pathogenesis. 112 |
| ISSN: | 1096-1208 |
| Popis: | In recent years Epithelial Mesenchymal Transition (EMT) has been proposed as a mechanism indispensable to acquisition of metastatic properties by tumor cells. In this study we tested the ability of Farrerol, a Chinese herb-derived compound to ablate the EMT in human lung squamous cell carcinoma cells. Human lung squamous cell carcinoma cells, Calu-1 were treated with various concentrations of Farrerol for 24 h to examine its effect on their viability by the MTT assay. Only those concentrations which showed least effect on the viability of Calu-1 cells were further used to evaluate the expression of epithelial and mesenchymal markers by western blotting. Furthermore the effect of such concentrations on the migration and invasion of Calu-1 cells was determined by wound healing and transwell invasion assays respectively. The results demonstrated that Farrerol treatment led to the downregulation of Slug and Zeb-1, transcriptional regulators of EMT with the concomitant increase and decrease in the expression of E-cadherin and vimentin respectively. These data were further supported by migration and invasion assays which demonstrated that Farrerol treatment caused inhibited the migration and invasion of Calu-1 lung squamous cell carcinoma cells. Taken together, our results indicate that Farrerol suppresses lung squamous cell carcinoma cell metastatic potential by modulating the expression of EMT proteins. |
| Databáze: | OpenAIRE |
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