Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach
| Autor: | Xiao-Dong Zhu, Song Qu, Fanyan Zeng, Xiao-Yu Li, Hui-Ling Meng, Zhong-Guo Liang, Ling Li, Xin-Bin Pan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Proteomics Pathology medicine.medical_specialty calmodulin Gene Expression Biology Tandem mass tag Tandem mass spectrometry Oxidative Phosphorylation Pathogenesis 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Biopsy Genetics medicine Biomarkers Tumor Humans recurrent nasopharyngeal carcinoma Chromatography High Pressure Liquid Neoplasm Staging Nasopharyngeal Carcinoma Oncogene medicine.diagnostic_test Carcinoma Cancer Nasopharyngeal Neoplasms General Medicine Articles Middle Aged medicine.disease Molecular medicine Neoplasm Proteins tandem mass tags 030104 developmental biology Nasopharyngeal carcinoma 030220 oncology & carcinogenesis Cancer research biomarker Female Neoplasm Recurrence Local |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X |
| Popis: | To date, there are no serum biomarkers available for the prediction of recurrent nasopharyngeal carcinoma (rNPC). The diagnosis of rNPC mostly depends on imaging and biopsy of diseased tissue; however, both of these methods work mostly if the target tumor is at an advanced stage. Therefore, the identificaqtion of recurrent biomarkers is urgently required. In the present study, we used tandem mass tag (TMT) labeling and high performance liquid chromatography (HPLC) fractionation followed by liquid chromatography-tandem mass spectrometry (LC‑MS/MS) to identify differentially expressed proteins. Serum was collected from 40 patients with NPC [recurrence (n=20) and no recurrence (n=20)]. Compared to non‑recurrent NPC (nrNPC), we found 59 proteins to be significantly dysregulated in rNPC; most of these have been previously reported to play a role in carcinogenesis. The dysregulation of calmodulin (CALM) was confirmed in 74 new patients [recurrence (n=32) and no recurrence (n=42)] by ELISA. Moreover, we performed a preliminary pathway analysis which revealed that oxidative phosphorylation was altered in the patients with rNPC compared to those with nrNPC. Taken together, these data identify a potential diagnostic biomarker for rNPC and elucidate the potential molecular mechanisms that are dysregulated and contribute to the pathogenesis of rNPC. |
| Databáze: | OpenAIRE |
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