Effects of Human Placental Amnion Derived Mesenchymal Stem Cells on Proliferation and Apoptosis Mechanisms in Chronic Kidney Disease in the Rat
| Autor: | Busra Cetinkaya, Dijle Kipmen-Korgun, Sadi Köksoy, Emin Turkay Korgun, Gozde Unek |
|---|---|
| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi |
| Rok vydání: | 2019 |
| Předmět: |
Chronic kidney failure
Proliferation Renal function Apoptosis Human amnion derived mesenchymal stem cells Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fibrosis medicine Sirius Red 030304 developmental biology 0303 health sciences Creatinine Kidney Amnion business.industry Mesenchymal stem cell Cell Biology medicine.disease medicine.anatomical_structure chemistry Rat Original Article business 030217 neurology & neurosurgery Developmental Biology Kidney disease |
| Zdroj: | International Journal of Stem Cells |
| ISSN: | 2005-5447 |
| DOI: | 10.15283/ijsc18067 |
| Popis: | Background and Objectives: The feature of chronic kidney failure (CKF) is loss of kidney functions due to erosion of healthy tissue and fibrosis. Recent studies showed that Mesenchymal stem cells (MSCs) differentiated into tubular epithelial cells thus renal function and structures renewed.Furthermore, MSCs protect renal function in CKF. Therefore, we aimed to investigate whether human amnion-derived mesenchymal stem cells (hAMSCs) can repair fibrosis and determine the effects on proliferation and apoptosis mechanisms in chronic kidney failure. Methods and Results: In this study, rat model of CKF was constituted by applying Aristolochic acid (AA). hAMSCs were isolated from term placenta amnion membrane and transplanted into tail vein of rats. At the end of 30 days and 60 days of recovery period, we examined expressions of PCNA, p57 and Parp-1 by western blotting. Immunoreactivity of PCNA, Ki67, IL-6 and Collagen type I were detected by immunohistochemistry. Besides, apoptosis was detected by TUNEL. Serum creatinine and urea were measured. Expressions of PCNA and Ki67 increased in hAMSC groups compared with AA group. Furthermore, expressions of PARP-1 apoptosis marker and p57 cell cycle inhibitory protein increased in AA group significantly according to control, hAMSC groups and sham groups. IL-6 proinflammatory cytokine increased in AA group significantly according to control, hAMSCs groups and sham groups. Expressions of Collagen type I protein reduced in hAMSCs groups compared to AA group. After hAMSC treatment, serum creatinine and urea levels significantly decreased compared to AA group. After injection of hAMSC to rats, Masson's Trichrome and Sirius Red staining showed fibrosis reduction in kidney. Conclusions: According to our results hAMSCs can be ameliorate renal failure. © 2019 by the Korean Society for Stem Cell Research. TYL-2014-131 This study was supported by Research Foundation of Akdeniz University, Turkey (project number: TYL-2014-131). |
| Databáze: | OpenAIRE |
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