Male reproductive traits, semen cryopreservation, and heterologous in vitro fertilization in the bobcat (Lynx rufus)
| Autor: | J.M. Aguilar, Eduardo R. S. Roldan, Natalia Gañán, Iñigo Sánchez, Raquel Ibáñez González, José Julián Garde, Montserrat Gomendio, A. Sestelo |
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| Přispěvatelé: | Consejo Superior de Investigaciones Científicas (España), Fundación BBVA, Wolfson Foundation, Ministerio de Medio Ambiente (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system media_common.quotation_subject medicine.medical_treatment Semen Fertilization in Vitro Biology Cryopreservation Andrology Human fertilization Cryoprotective Agents Food Animals medicine Animals Small Animals reproductive and urinary physiology Sperm motility Cells Cultured media_common In vitro fertilisation urogenital system Equine Reproduction Semen cryopreservation Sperm Semen Analysis Phenotype Lynx Cats Animal Science and Zoology Female Seasons Semen Preservation |
| Popis: | There is limited information on bobcat ejaculate traits and sperm cryopreservation and fertilizing ability. Bobcats were electroejaculated under general anesthesia in November (autumn) and April (spring), and endocrine and sperm traits were characterized. Testosterone (mean ± SEM: 0.90 ± 0.15 ng/mL) was not different between sampling times, but cortisol (average: 13.95 ± 1.73 μg/dL) was significantly higher in April. Average number of spermatozoa was 10.0 ± 3.4 × 106 sperm/ejaculate, with values being significantly higher in April. Sperm motility (average 55.7 ± 5.8% motile sperm) was not different between sampling times. The proportion of normal spermatozoa in the ejaculate (average: 14.7 ± 2.1%) was significantly higher in April, but the percentage of spermatozoa with intact acrosomes (average: 43.7 ± 3.8%) was significantly higher in autumn. Spermatozoa were cryopreserved in a Tes-Tris–based diluent (TEST) or Biladyl, both containing 20% egg yolk and 4% glycerol. Diluted sperm were loaded into straws, refrigerated using a programmable thermoblock with a dry chamber, frozen in nitrogen vapors, thawed, and incubated in F-10 medium with 5% fetal bovine serum for up to 3 h. After cryopreservation in TEST, there were about 50% motile sperm upon thawing, and survival was high during incubation post-thaw. Cryopreservation in Biladyl led to similar results, but motility decreased substantially during incubation post-thaw. Bobcat spermatozoa fertilized domestic cat oocytes matured in vitro. Fertilization rates were higher for sperm collected in April and cryopreserved in TEST (46%) than for those cryopreserved using Biladyl ( Natalia Gañán enjoyed scholarships from CSIC (I3P programme) and the BBVA Foundation. Raquel González enjoyed a scholarship from CSIC (I3P programme). Adrián Sestelo received a BBVA Foundation scholarship. E.R.S. Roldan is the recipient of a Royal Society Wolfson Research Merit Award. Funding from the Spanish Ministry of the Environment, CSIC, and the BBVA Foundation is gratefully acknowledged. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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