Deletion of psbQ’ gene in Cyanidioschyzon merolae reveals the function of extrinsic PsbQ’ in PSII
| Autor: | Anna Golke, Elżbieta Romanowska, Wioleta Wasilewska, Maksymilian Zienkiewicz, Anna Drożak, Tomasz Krupnik |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DTA toxin Chloramphenicol O-Acetyltransferase Stable red algae nuclear transformation Protein subunit Cyanidioschyzon merolae Mutant Plant Science macromolecular substances Article PSII extrinsic protein deletion 03 medical and health sciences PSII mutants PsbQ’ Oxygen evolving activity Chloramphenicol acetyltransferase Genetics Photosynthesis Gene Selectable marker biology food and beverages Photosystem II Protein Complex General Medicine biology.organism_classification PEG Cell biology 030104 developmental biology Rhodophyta Phycobilisome Agronomy and Crop Science Function (biology) |
| Zdroj: | Plant Molecular Biology |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | Key message We have successfully produced single-cell colonies of C. merolae mutants, lacking the PsbQ’ subunit in its PSII complex by application of DTA-aided mutant selection. We have investigated the physiological changes in PSII function and structure and proposed a tentative explanation of the function of PsbQ’ subunit in the PSII complex. Abstract We have improved the selectivity of the Cyanidioschyzon merolae nuclear transformation method by the introduction of diphtheria toxin genes into the transformation vector as an auxiliary selectable marker. The revised method allowed us to obtained single-cell colonies of C. merolae, lacking the gene of the PsbQ’ extrinsic protein. The efficiency of gene replacement was extraordinarily high, allowing for a complete deletion of the gene of interest, without undesirable illegitimate integration events. We have confirmed the absence of PsbQ’ protein at genetic and protein level. We have characterized the physiology of mutant cells and isolated PSII protein complex and concluded that PsbQ’ is involved in nuclear regulation of PSII activity, by influencing several parameters of PSII function. Among these: oxygen evolving activity, partial dissociation of PsbV, regulation of dimerization, downsizing of phycobilisomes rods and regulation of zeaxanthin abundance. The adaptation of cellular physiology appeared to favorite upregulation of PSII and concurrent downregulation of PSI, resulting in an imbalance of energy distribution, decrease of photosynthesis and inhibition of cell proliferation. Electronic supplementary material The online version of this article (10.1007/s11103-017-0685-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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