Microarrays of bladder cancer tissue are highly representative of proliferation index and histological grade
| Autor: | Antonio Nocito, Thomas Forster, Peter Jäger, Guido Sauter, Hartmut Knönagel, Katrin Süess, Renata Flury, Marcus Rist, James Bruderer, Jean Luc Fehr, Manuel Anabitarte, Michael J. Mihatsch, Peter Schraml, Göran Alund, André Fijan, Urs Wagner, Andreas J. Schoenenberger, Juha Kononen, Ulrico Schmid, Franz Hering, Lukas Bubendorf, Robert Maurer, Holger Moch, Thomas Hardmeier, Eva Maria Tinner, Thomas C. Gasser, Daniel Ackermann |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Proliferation index Adenocarcinoma Pathology and Forensic Medicine Carcinoma Adenosquamous medicine Humans Clinical significance Carcinoma Small Cell Aged Oligonucleotide Array Sequence Analysis Retrospective Studies Carcinoma Transitional Cell Chi-Square Distribution Bladder cancer Tissue microarray Urinary bladder business.industry Sarcoma Middle Aged Prognosis medicine.disease Immunohistochemistry Survival Analysis Ki-67 Antigen Transitional cell carcinoma medicine.anatomical_structure Urinary Bladder Neoplasms Carcinoma Squamous Cell Female DNA microarray business Follow-Up Studies |
| Zdroj: | The Journal of Pathology. 194:349-357 |
| ISSN: | 0022-3417 |
| DOI: | 10.1002/1096-9896(200107)194:3<349::aid-path887>3.0.co;2-d |
| Popis: | The number of genes suggested to play a role in cancer biology is rapidly increasing. To be able to test a large number of molecular parameters in sufficiently large series of primary tumours, a tissue microarray (TMA) approach has been developed where samples from up to 1000 tumours can be simultaneously analysed on one glass slide. Because of the small size of the individual arrayed tissue samples (diameter 0.6 mm), the question arises of whether these specimens are representative of their donor tumours. To investigate how representative are the results obtained on TMAs, a set of 2317 bladder tumours that had been previously analysed for histological grade and Ki67 labelling index (LI) was used to construct four replica TMAs from different areas of each tumour. Clinical follow-up information was available from 1092 patients. The histological grade and the Ki67 LI were determined for every arrayed tumour sample (4x2317 analyses each). Despite discrepancies in individual cases, the grade and Ki67 information obtained on minute arrayed samples were highly similar to the data obtained on large sections (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text