Whole‐genome sequencing revealsListeria monocytogenesdiversity and allows identification of long‐term persistent strains in Brazil

Autor: Marc Lecuit, Srinand Sreevatsan, Douglas R. Call, Alexandra Moura, Joshua J. Woodward, Adelle P. McFarland, Johannetsy J. Avillan, Nicole Herman, Luís Augusto Nero, Anderson Carlos Camargo
Přispěvatelé: Universidade Federal de Vicosa (UFV), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Paul G. Allen School for Global Animal Health [WSU, USA], Washington State University (WSU), University of Minnesota [Twin Cities] (UMN), University of Minnesota System, University of Washington [Seattle], Michigan State University [East Lansing], Michigan State University System, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), The present material is based upon work supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)., The authors express their gratitude to Deyse Vallim and Ernesto Hofer from the Laboratory of Bacterial Zoonoses (FioCruz, Brazil) for provision of bacterial strains from CLIST collection., Universidade Federal de Viçosa = Federal University of Viçosa (UFV), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), DIAKITE, andrée
Rok vydání: 2019
Předmět:
Meat
Virulence
Biology
medicine.disease_cause
Microbiology
Genome
Article
resistance
03 medical and health sciences
Listeria monocytogenes
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
medicine
Humans
Listeriosis
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Gene
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
[SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Whole genome sequencing
Genetics
whole genome sequencing
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
0303 health sciences
Genetic diversity
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
030306 microbiology
molecular typing
Genetic Variation
persistence
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Stop codon
virulence
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Food Microbiology
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Multilocus sequence typing
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Brazil
Genome
Bacterial
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Multilocus Sequence Typing
Zdroj: Environmental Microbiology
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, 21 (12), pp.4478-4487. ⟨10.1111/1462-2920.14726⟩
Environmental Microbiology, 2019, 21 (12), pp.4478-4487. ⟨10.1111/1462-2920.14726⟩
Environ Microbiol
ISSN: 1462-2920
1462-2912
Popis: International audience; Advances in whole-genome sequencing (WGS) technologies have documented genetic diversity and epidemiology of the major foodborne pathogen Listeria monocytogenes (Lm) in Europe and North America, but data concerning South America are scarce. Here, we examined the population structure and genetic diversity of this major foodborne pathogen collected in Brazil. Based on core genome multilocus sequence typing (cgMLST), isolates from lineages I (n = 22; 63%) and II (n = 13; 37%) were distributed into 10 different sublineages (SLs) and represented 31 new cgMLST types (CTs). The most prevalent SLs were SL9 (n = 9; 26%), SL3 (n = 6; 17%) and SL2 and SL218 (n = 5; 14%). Isolates belonging to CTs L2-SL9-ST9-CT4420 and L1-SL315-ST520-CT4429 were collected 3 and 9 years apart, respectively, revealing long-term persistence of Lm in Brazil. Genetic elements associated with stress survival were present in 60% of isolates (57% SSI-1 and 3% SSI-2). Pathogenic islands were present in 100% (LIPI-1), 43% (LIPI-3) and 6% (LIPI-4) of the isolates. Mutations leading to premature stop codons were detected in the prfA and inlA virulence genes. This study is an important contribution to understanding the genomic diversity and epidemiology of Lm in South America. In addition, the results highlight the importance of using WGS to reveal Lm long-term persistence.
Databáze: OpenAIRE
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