Evidences Suggesting that Distinct Immunological and Cellular Responses to Light Damage Distinguishes Juvenile and Adult Rat Retinas

Autor: Shasha Lv, Laura-Alexie Chevrolat, Hyba Bessaklia, Wassila Ait Igrine, Anna Polosa, Pierre Lachapelle
Rok vydání: 2019
Předmět:
0301 basic medicine
Light
Fluorescent Antibody Technique
immune response
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
medicine.diagnostic_test
Age Factors
juvenile and adult rats
General Medicine
Diabetic retinopathy
Immunohistochemistry
Pathophysiology
Computer Science Applications
intrinsic resistance
medicine.anatomical_structure
Retinopathy
retinal structure and function
medicine.medical_specialty
neurotrophic factors
Biology
Article
Retina
Catalysis
Inorganic Chemistry
03 medical and health sciences
Retinal Diseases
Internal medicine
Retinitis pigmentosa
Electroretinography
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
Organic Chemistry
Retinal
medicine.disease
Electrophysiological Phenomena
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
light-induced retinopathy
030221 ophthalmology & optometry
Biomarkers
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 11
International Journal of Molecular Sciences, Vol 20, Iss 11, p 2744 (2019)
ISSN: 1422-0067
Popis: To unravel the mechanisms behind the higher resistance to light damage of juvenile (JR) versus adult (AR) rats, Sprague Dawley rats were exposed to a bright luminous environment of 10, 000 lux. The light-induced retinopathy (LIR) was assessed with histology, electroretinography and immunohistochemistry (IHC). In JR, 2 days of exposure induced the typical LIR, while >
3 days added little LIR. IHC revealed a subtle migration of microglia (Iba1 marker) from the inner to the outer retina after 3 days of exposure in JR contrasting with the stronger reaction seen after 1 day in AR. Similarly, in JR, the Mü
ller cells expressed less intense GFAP, CNTF and FGF2 staining compared to AR. Our results suggest that in JR the degree of retinal damage is not proportional to the duration of light exposure (i.e., dose-independent retinopathy), contrasting with the dose-dependent LIR reported in AR. The immature immune system in JR may explain the delayed and/or weaker inflammatory response compared to AR, a finding that would also point to the devastating contribution of the immune system in generating the LIR phenotype, a claim also advanced to explain the pathophysiology of other retinal degenerative disorders such as Age-related Macular Degeneration, Diabetic Retinopathy and Retinitis Pigmentosa.
Databáze: OpenAIRE
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