Evidences Suggesting that Distinct Immunological and Cellular Responses to Light Damage Distinguishes Juvenile and Adult Rat Retinas
Autor: | Shasha Lv, Laura-Alexie Chevrolat, Hyba Bessaklia, Wassila Ait Igrine, Anna Polosa, Pierre Lachapelle |
---|---|
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Light Fluorescent Antibody Technique immune response lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine lcsh:QH301-705.5 Spectroscopy medicine.diagnostic_test Age Factors juvenile and adult rats General Medicine Diabetic retinopathy Immunohistochemistry Pathophysiology Computer Science Applications intrinsic resistance medicine.anatomical_structure Retinopathy retinal structure and function medicine.medical_specialty neurotrophic factors Biology Article Retina Catalysis Inorganic Chemistry 03 medical and health sciences Retinal Diseases Internal medicine Retinitis pigmentosa Electroretinography medicine Animals Physical and Theoretical Chemistry Molecular Biology Organic Chemistry Retinal medicine.disease Electrophysiological Phenomena Rats Disease Models Animal 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 chemistry light-induced retinopathy 030221 ophthalmology & optometry Biomarkers |
Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 11 International Journal of Molecular Sciences, Vol 20, Iss 11, p 2744 (2019) |
ISSN: | 1422-0067 |
Popis: | To unravel the mechanisms behind the higher resistance to light damage of juvenile (JR) versus adult (AR) rats, Sprague Dawley rats were exposed to a bright luminous environment of 10, 000 lux. The light-induced retinopathy (LIR) was assessed with histology, electroretinography and immunohistochemistry (IHC). In JR, 2 days of exposure induced the typical LIR, while > 3 days added little LIR. IHC revealed a subtle migration of microglia (Iba1 marker) from the inner to the outer retina after 3 days of exposure in JR contrasting with the stronger reaction seen after 1 day in AR. Similarly, in JR, the Mü ller cells expressed less intense GFAP, CNTF and FGF2 staining compared to AR. Our results suggest that in JR the degree of retinal damage is not proportional to the duration of light exposure (i.e., dose-independent retinopathy), contrasting with the dose-dependent LIR reported in AR. The immature immune system in JR may explain the delayed and/or weaker inflammatory response compared to AR, a finding that would also point to the devastating contribution of the immune system in generating the LIR phenotype, a claim also advanced to explain the pathophysiology of other retinal degenerative disorders such as Age-related Macular Degeneration, Diabetic Retinopathy and Retinitis Pigmentosa. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |