Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
| Autor: | Laura Valenzuela, Iván López-Montero, Ana Iglesias-Juez, Julia Alvarez-Malmagro, Marcos Pita, Cristina Gutierrez-Sanchez, Gabriel García-Molina, Marisela Vélez, Antonio L. De Lacey, Paolo Natale |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Fundación Ramón Areces, Comunidad de Madrid, Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2019 |
| Předmět: |
Biomimetic membranes
ATPase Potentiometric titration Potentiometric biosensor Lipid Bilayers Biophysics 02 engineering and technology Biosensing Techniques 01 natural sciences Redox chemistry.chemical_compound Adenosine Triphosphate Limit of Detection Electrochemistry Escherichia coli Sulfhydryl Compounds Physical and Theoretical Chemistry Lipid bilayer Electrochemical gradient Electrodes Adenosine Triphosphatases Aniline Compounds ATP synthase biology ATP-synthase 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology Enzymes Immobilized 0104 chemical sciences ATP chemistry biology.protein Potentiometry Gold Protons 0210 nano-technology Adenosine triphosphate Biosensor |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1878-562X 2018-0950 |
| Popis: | [EN] Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We report a potentiometric biosensor based on ATP-synthase from Escherichia coli reconstituted in a floating phospholipid bilayer over gold electrodes modified with a 4-aminothiophenol self-assembled monolayer. The use of a pH-dependent redox probe on the electrode surface allows a simple, specific and reliable on site determination of ATP concentration from 1 μM to 1 mM. The broad range ATP biosensor can offer an alternative way of measuring in a few minutes the presence of microbial contamination. A.L.D, M.P. and M.V. thank MCIU/AEI/FEDER, EU for funding project RTI2018-095090-B-I00. L.V. and A.I.J. thank Fundación Ramón Areces for financial support through the project OTR02666 and Prof. Rosal García (University of Alcalá) for the use of his laboratory facilities. I.L.-M. and M.V. thank the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA-4403 SINOXPHOS-CM). G.G.M. thanks MCIU/AEI/FEDER, EU for a predoctoral contract (BES-2016-078815). |
| Databáze: | OpenAIRE |
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