PBDX is the XG blood group gene
| Autor: | Polly A. Weller, Nathan A. Ellis, James German, Tian Z. Ye, Peter N. Goodfellow, Patricia Tippett, Stephen G. Thomas, A. C. Petty, George Banting, Marion E. Reid |
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| Rok vydání: | 1994 |
| Předmět: |
Genetic Linkage
medicine.drug_class Blotting Western Molecular Sequence Data Peptide 12E7 Antigen Cross Reactions Monoclonal antibody Polymerase Chain Reaction Epitope Epitopes Mice Antigen Antigens CD Tumor Cells Cultured Genetics medicine Animals Humans Amino Acid Sequence RNA Messenger Peptide sequence chemistry.chemical_classification Membrane Glycoproteins Base Sequence Sequence Homology Amino Acid biology Antibodies Monoclonal Hemagglutination Tests Fibroblasts Hematopoietic Stem Cells Virology Molecular biology Blot Genes chemistry Organ Specificity Polyclonal antibodies Blood Group Antigens biology.protein Rabbits Antibody Cell Adhesion Molecules |
| Zdroj: | Nature Genetics. 8:285-290 |
| ISSN: | 1546-1718 1061-4036 |
| Popis: | We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a-) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG. |
| Databáze: | OpenAIRE |
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