HYDROLYSIS OF CONJUGATED ESTROGEN FRACTIONS IN HUMAN PREGNANCY URINE
| Autor: | Sidsel Bugge, Mona Nilsen, Ann Metcalfe-Gibson, R. Hobkirk |
|---|---|
| Rok vydání: | 1961 |
| Předmět: |
medicine.drug_class
Estrone chemistry.chemical_compound Hydrolysis Estradiol Congeners Pregnancy Enzymatic hydrolysis Contraceptive Agents Female medicine Humans chemistry.chemical_classification Estrogens Conjugated (USP) biology Estrogens Estriol General Medicine Helix pomatia biology.organism_classification Body Fluids Enzyme chemistry Biochemistry Estrogen Female Acid hydrolysis |
| Zdroj: | Canadian Journal of Biochemistry and Physiology. 39:1501-1514 |
| ISSN: | 0576-5544 |
| DOI: | 10.1139/o61-162 |
| Popis: | The release of six estrogen fractions from conjugation in human pregnancy urines has been studied using various hydrolytic methods. The estrogens concerned were estrone, estradiol-17β (estradiol), 2-methoxyestrone, 16-epiestriol, and a ring D ketolic fraction (mainly 16α-hydroxyestrone). Considerable amounts of urinary estrone and ring D ketolic estrogens may be conjugated in a non-glucuronide form. In these cases an enzyme preparation containing β-glucuronidase and sulphatase, prepared from the digestive juice of the snail Helix pomatia, proved to be superior to β-glucuronidase enzymes of bacterial or mammalian liver origin. Conventional hot acid hydrolysis yielded levels of estrone, estradiol, estriol, and 16-epiestriol which agreed fairly well with those obtained following snail enzyme hydrolysis. In some urines, hot acid treatment was not suitable for hydrolysis of conjugated 2-methoxyestrone. Optimum hydrolytic conditions for both normal and diabetic pregnancy urines were realized by incubating for 24 hours with 500 units of the snail β-glucuronidase and 250 units of sulphatase/ml of urine at pH 5.2 and 37–38 °C. |
| Databáze: | OpenAIRE |
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