Retracted : MicroRNA‐520a‐3p suppresses epithelial–mesenchymal transition, invasion, and migration of papillary thyroid carcinoma cells via the JAK1 ‐mediated JAK/STAT signaling pathway
| Autor: | Bo Li, Min Guo, Chang‐Long Bi, Yili Fu, Ying-qi Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male STAT3 Transcription Factor 0301 basic medicine Epithelial-Mesenchymal Transition endocrine system diseases Physiology Clinical Biochemistry Apoptosis Vimentin Papillary thyroid cancer Young Adult 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor microRNA medicine Humans Gene silencing Neoplasm Invasiveness Thyroid Neoplasms Epithelial–mesenchymal transition STAT3 Cell Proliferation biology Chemistry JAK-STAT signaling pathway Janus Kinase 1 Cell Biology Middle Aged Cell cycle medicine.disease G1 Phase Cell Cycle Checkpoints Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Thyroid Cancer Papillary 030220 oncology & carcinogenesis biology.protein Cancer research Female Signal Transduction |
| Zdroj: | Journal of Cellular Physiology. 234:4054-4067 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.27199 |
| Popis: | Papillary thyroid cancer (PTC) is a kind of thyroid cancer and frequently presents with epithelial-mesenchymal transition (EMT). MicroRNAs (miRNAs) were previously reported to be associated with PTC. Thus, this study aims to define the role of microRNA-520a-3p (miR-520a-3p) in PTC through the JAK/STAT signaling pathway by targeting JAK1. The PTC and normal thyroid tissues of 137 PTC patients were collected. First of all, the expression pattern of miR-520a-3p, JAK1, JAK2, STAT3, E-cadherin, and vimentin in PTC was identified. The relationship between miR-520a-3p and JAK1 was predicted and analyzed. And a series of miR-520a-3p mimic or inhibitor, or siRNA JAK1 introduced into PTC cells were applied to examine the effect of miR-520a-3p on PTC cell viability, migration, invasion, cell cycle, apoptosis, and EMT. Meanwhile, the regulatory effect of miR-520a-3p and JAK1 on the JAK/STAT signaling pathway was also determined. The expression of JAK1, JAK2, STAT3, and vimentin increased yet miR-520a-3p and E-cadherin decreased in PTC tissue. JAK1 was negatively regulated by miR-520a-3p. Functionally, EMT induction was prevented by miR-520a-3p upregulation through downregulating JAK1. When upregulating miR-520a-3p or silencing JAK1 in PTC cells, PTC cell viability, migration, and invasion were inhibited yet cell apoptosis promoted with cells arrested at G1 phase, indicating that miR-520a-3p prevented PTC progression by downregulating JAK1. Moreover, miR-520a-3p elevation or JAK1 inhibition inactivated the JAK/STAT signaling pathway. Collectively, miR-520a-3p prevents cancer progression through inactivating the JAK/STAT signaling pathway by downregulating JAK1 in PTC. |
| Databáze: | OpenAIRE |
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