An animal model for allergic penicilliosis induced by the intranasal instillation of viable Penicillium chrysogenum conidia
| Autor: | J D Cooley, Cynthia A. Jumper, D C Straus, W C Wong, J C Hutson, H J Williams, Christopher J. Schwab |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Allergy Sick Building Syndrome Time Factors medicine.medical_treatment Penicillium chrysogenum Gastroenterology Penicilliosis Interferon-gamma Mice Internal medicine medicine Eosinophilia Animals Administration Intranasal Lung medicine.diagnostic_test biology business.industry Original Articles respiratory system Immunoglobulin E biology.organism_classification medicine.disease Neutrophilia Eosinophils Mice Inbred C57BL Bronchoalveolar lavage medicine.anatomical_structure Cytokine Immunoglobulin G Immunology Female Interleukin-4 medicine.symptom business Bronchoalveolar Lavage Fluid |
| Popis: | BACKGROUND—A study was undertaken to determine the consequences of long term intranasal instillation of Penicillium chrysogenum propagules in a mouse model. METHODS—C57 Black/6 mice were inoculated intranasally each week for six weeks with 104 viable and non-viable P chrysogenum conidia. Cytokine levels and cellular responses in these animals were then measured. RESULTS—Compared with controls, mice inoculated intranasally each week for six weeks with 104P chrysogenum conidia (average viability 25%) produced significantly more total serum IgE (mean difference 1823.11, lower and upper 95% confidence intervals (CI) 539.09 to 3107.13), peripheral eosinophils (mean difference 5.11, 95% CI 2.24 to 7.99), and airway eosinophilia (rank difference 11.33, 95% CI 9.0 to 20.0). With the exception of airway neutrophilia (mean difference 20.89, 95% CI 3.72 to 38.06), mice inoculated intranasally with 104 non-viable conidia did not show significant changes in total serum IgE, peripheral or airway eosinophils. However, when compared with controls, this group (104 non-viable) had a significant increase in total serum IgG2a (mean difference 1990.56, 95% CI 790.48 to 3190.63) and bronchoalveolar lavage (BAL) fluid levels of interferon (IFN)-γ (mean difference 274.72, 95% CI 245.26 to 304.19). In addition, lung lavages from mice inoculated intranasally with 104 viable P chrysogenum conidia had significantly increased levels of interleukin (IL)-4 (mean difference 285.28, 95% CI 108.73 to 461.82) and IL-5 (mean difference 16.61, 95% CI 11.23 to 21.99). The IgG2a/IgE ratio and the IFN-γ/IL-4 ratio was lower in the group of mice inoculated intranasally with 104 viable conidia than in the 104 non-viable conidia group and the controls. When proteins were extracted from P chrysogenum conidia, attached to microtitre plates and incubated with serum from the 104 viable group, significant increases in conidia-specific IgE and IgG1 were observed compared with controls, while serum from the 104 non-viable group was similar to controls. CONCLUSIONS—These data suggest that long term inhalation of viable P chrysogenum propagules induces type 2 T helper cell mediated (Th2) inflammatory responses such as increases in total and conidia-specific serum IgE and IgG1, together with BAL fluid levels of IL-4 and IL-5 and peripheral and airway eosinophilia, which are mediators of allergic reactions. |
| Databáze: | OpenAIRE |
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