A targeted ultra performance liquid chromatography – Tandem mass spectrometric assay for tyrosine and metabolites in urine and plasma: Application to the effects of antibiotics on mice
| Autor: | Dumas, Marc-Emmanuel, Letertre, Marine, Myridakis, Antonis, Whiley, Luke, Camuzeaux, Stéphane, Lewis, Matthew, Chappell, Katie, Thaikkatil, Annie, Nicholson, Jeremy, Swann, Jonathan, Wilson, Ian, Letertre, Marine P.M. |
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| Přispěvatelé: | Division of Computational and Systems Medicine, Imperial College London, London, SW7 2AZ, UK, Imperial College London - National Heart and Lung Institute, Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Imperial College London, Institute for Women's Health [London], University College London Hospitals (UCLH), Department of Surgery and Cancer [Londres, Royaume-Uni], Biomolecular Medicine, Division of Computational and Systems Medicine, Imperial College London, London, SW7 2AZ, UK., Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Electrospray
Clinical Biochemistry Urine 030226 pharmacology & pharmacy 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry MESH: Tyrosine MESH: Linear Models Cresols Mice chemistry.chemical_compound 0302 clinical medicine Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Metabolites MESH: Animals Tyrosine Chromatography High Pressure Liquid Dansyl Compounds Mice Inbred BALB C Dansyl chloride General Medicine Anti-Bacterial Agents MESH: Reproducibility of Results MESH: Cresols Female Glucuronide P-cresol conjugates MESH: Mice Inbred BALB C MESH: Limit of Detection MESH: Gastrointestinal Microbiome Tyrosinemia 03 medical and health sciences [CHIM.ANAL]Chemical Sciences/Analytical chemistry Quantification MESH: Anti-Bacterial Agents Biofluids medicine Animals MESH: Chromatography High Pressure Liquid MESH: Mice MESH: Dansyl Compounds Chromatography 010401 analytical chemistry Reproducibility of Results MESH: Tandem Mass Spectrometry Cell Biology medicine.disease Gastrointestinal Microbiome 0104 chemical sciences chemistry Linear Models MESH: Female [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2021, 1164, pp.122511. ⟨10.1016/j.jchromb.2020.122511⟩ Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2021, 1164, pp.122511. ⟨10.1016/j.jchromb.2020.122511⟩ |
| ISSN: | 1570-0232 1873-376X |
| Popis: | International audience; Tyrosine plays a key role in mammalian biochemistry and defects in its metabolism (e.g., tyrosinemia, alkaptonuria etc.) have significant adverse consequences for those affected if left untreated. In addition, gut bacterially-derived p-cresol and its metabolites are of interest as a result of various effects on host xenobiotic metabolism. A fit-for-purpose quantitative ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay was developed to target and quantify tyrosine and eleven metabolites in urine and plasma. Dansylation, using dansyl chloride, was used to improve chromatographic and mass spectral properties for tyrosine and nine phenolic metabolites, with detection using positive electrospray ionisation (ESI). The sulfate and glucuronide conjugates of p-cresol, where the phenol group was blocked, were quantified intact, using negative ESI via polarity switching during the same run. Sample preparation for urine and plasma involved deproteinization by solvent precipitation (of acetonitrile:isopropyl alcohol (1:1 v/v)) followed by in situ dansylation in 96 well plates. To minimize sample and solvent usage, and maximize sensitivity, analysis was performed using microbore reversed-phase gradient UPLC on a C8 phase with a 7.5 min. cycle time. The coefficients of variation obtained were |
| Databáze: | OpenAIRE |
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