Early physiological and biological features in three animal models of induced acute lung injury
| Autor: | Octavi Martí-Sistac, María Elisa Quílez, Carlos Flores, Lluís Blanch, Jesús Villar, Ferranda Puig, Antonio Artigas, Gemma Fuster, Josefina López-Aguilar, Carolina García-Martín |
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| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
Male Pathology medicine.medical_specialty Time Factors Context (language use) Pulmonary compliance Lung injury Critical Care and Intensive Care Medicine Severity of Illness Index Positive-Pressure Respiration Rats Sprague-Dawley Random Allocation Intensive care medicine Animals Respiratory system Lung business.industry Respiratory disease respiratory system medicine.disease Respiration Artificial Endotoxemia Rats respiratory tract diseases Disease Models Animal medicine.anatomical_structure Brain Injuries Acute Disease Metalloproteases Breathing business |
| Zdroj: | Intensive Care Medicine. 36:347-355 |
| ISSN: | 1432-1238 0342-4642 |
| Popis: | Critically ill patients often develop acute lung injury (ALI) in the context of different clinical conditions. We aimed to explore differences in early local and systemic features in three experimental animal models of ALI. Mechanically ventilated male Sprague–Dawley rats were randomized to high tidal volume (VT) ventilation (HVT) (n = 8, VT 24 ml/kg), massive brain injury (MBI) (n = 8, VT 8 ml/kg) or endotoxemia (LPS) (n = 8, VT 8 ml/kg). Each experimental group had its own control group of eight rats (VT 8 ml/kg). We measured arterial blood gases, mean arterial pressure, lung compliance, inflammatory mediators in plasma and their expression and gelatinase activity in the lungs after 3 h of injury. Despite maintaining relatively normal lung function without evidence of important structural changes, we observed altered lung and systemic inflammatory responses in all three experimental models. LPS triggered the most robust inflammatory response and HVT the lowest systemic proinflammatory response. The HVT group had higher Il6, Tnf and Cxcl2 mRNA in lungs than MBI animals. Metalloproteinase activity/expression and neutrophilic recruitment in the lungs were higher in HVT than in LPS or MBI. The early responses to direct or remote lung insult in our three models of ALI captured different physiological and biological features that could lead to respiratory and/or multiorgan failure. |
| Databáze: | OpenAIRE |
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