Ubisol Coenzyme Q10 promotes mitochondrial biogenesis in HT22 cells challenged by glutamate
| Autor: | Mia Hall, Qi Qi, P. Andy Li, Mary A Zimmerman, Guisheng Chen, Suresh L. Mehta |
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| Rok vydání: | 2020 |
| Předmět: |
Coenzyme Q10
Cancer Research Programmed cell death mitochondrial biogenesis Chemistry Glutamate receptor Excitotoxicity glutamate General Medicine Articles TFAM Peroxisome medicine.disease_cause Neuroprotection Cell biology chemistry.chemical_compound Immunology and Microbiology (miscellaneous) Mitochondrial biogenesis coenzyme Q10 medicine excitotoxicity |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 |
| Popis: | Background: Glutamate-induced excitotoxicity is a well-recognized cause of neuronal cell death and nutritional supplementation with Coenzyme Q10 (CoQ10) has previously been shown to have neuro-protective actions against it. The objective of this study was to determine whether the protective effect of CoQ10 against glutamate could be attributed to stimulating mitochondrial biogenesis. Results: The mouse hippocampal neuronal HT22 cells were incubated with glutamate with or without ubisol Q10 treatment. Significant deterioration of cells after glutamate exposure was observed under a light microscope and cell viability assay, along with a significant drop in clonogenic ability. Glutamate significantly decreased the mitochondrial biogenesis related protein levels of Akt, CREB, PGC-1α, and NRF2, and reduced mitochondrial biogenesis assessed by a mitochondrial biogenesis kit. Pretreatment with CoQ10 prevented the decreases of Akt, CREB, PGC-1α, and NRF2 and increased mitochondrial biogenesis. Conclusions: Taken together, these results describe a new mechanism of CoQ10-conveyed neuro-protection and indicate a central role for mitochondrial biogenesis in protecting against glutamate-induced excitotoxicity. |
| Databáze: | OpenAIRE |
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