In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy

Autor: Eric Peltier, Genevieve Bourg Heckly, Sophie Moreno-Swirc, Tom Vercauteren, Luc Thiberville, Charlotte Cave
Přispěvatelé: Service de pneumologie, oncologie thoracique et soins intensifs respiratoires [Rouen], Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU), Laboratoire de physicochimie biomoléculaire et cellulaire (LPBC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS), Hôpital Charles Nicolle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Université Paris 13 (UP13)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Breton, Céline
Rok vydání: 2006
Předmět:
Pulmonary and Respiratory Medicine
Diagnostic Imaging
Pathology
medicine.medical_specialty
MESH: Fiber Optic Technology
bronchoscopy
Confocal
MESH: Microscopy
Fluorescence
Bronchi
Biology
Critical Care and Intensive Care Medicine
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
law.invention
03 medical and health sciences
0302 clinical medicine
In vivo
Confocal microscopy
law
Intensive care
Fluorescence microscope
medicine
MESH: Microscopy
Confocal
Fiber Optic Technology
Humans
precancerous conditions
MESH: Bronchial Neoplasms
MESH: Humans
Microscopy
Confocal
MESH: Diagnostic Imaging
Bronchial Neoplasms
MESH: Bronchi
respiratory system
basement membrane
3. Good health
Autofluorescence
030228 respiratory system
confocal
Microscopy
Fluorescence
030220 oncology & carcinogenesis
microscopy
[SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
fluorescence
Preclinical imaging
Ex vivo
Zdroj: American Journal of Respiratory and Critical Care Medicine
American Journal of Respiratory and Critical Care Medicine, American Thoracic Society, 2007, 175 (1), pp.22-31. ⟨10.1164/rccm.200605-684OC⟩
American Journal of Respiratory and Critical Care Medicine, 2007, 175 (1), pp.22-31. ⟨10.1164/rccm.200605-684OC⟩
ISSN: 1073-449X
1535-4970
DOI: 10.1164/rccm.200605-684OC⟩
Popis: International audience; RATIONALE: Fibered confocal fluorescence microscopy (FCFM) is a new technique that produces microscopic imaging of a living tissue through a 1-mm fiberoptic probe that can be introduced into the working channel of the bronchoscope. OBJECTIVES: To analyze the microscopic autofluorescence structure of normal and pathologic bronchial mucosae using FCFM during bronchoscopy. METHODS: Bronchial FCFM and spectral analyses were performed at 488-nm excitation wavelength on two bronchial specimens ex vivo and in 29 individuals at high risk for lung cancer in vivo. Biopsies of in vivo FCFM-imaged areas were performed using autofluorescence bronchoscopy. RESULTS: Ex vivo and in vivo microscopic and spectral analyses showed that the FCFM signal mainly originates from the elastin component of the basement membrane zone. Five distinct reproducible microscopic patterns were recognized in the normal areas from the trachea down to the more distal respiratory bronchi. In areas of the proximal airways not previously biopsied, one of these patterns was found in 30 of 30 normal epithelia, whereas alterations of the autofluorescence microstructure were observed in 19 of 22 metaplastic or dysplastic samples, five of five carcinomas in situ, and two of two invasive lesions. Disorganization of the fibered network could be found on 9 of 27 preinvasive lesions, compatible with early disruptions of the basement membrane zone. FCFM alterations were also observed in a tracheobronchomegaly syndrome and in a sarcoidosis case. CONCLUSIONS: Endoscopic FCFM represents a minimally invasive method to study specific basement membrane alterations associated with premalignant bronchial lesions in vivo. The technique may also be useful to study the bronchial wall remodeling in nonmalignant chronic bronchial diseases.
Databáze: OpenAIRE
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