An inactivated Vero cell-grown Japanese encephalitis vaccine formulated with Advax, a novel inulin-based adjuvant, induces protective neutralizing antibody against homologous and heterologous flaviviruses
| Autor: | Hiroko Toriniwa, Nikolai Petrovsky, Peter D. Cooper, Mario Lobigs, Roy A. Hall, Tomoyoshi Komiya, Megan Pavy, Päivi Lobigs |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_treatment
viruses Cross Reactions Antibodies Viral 03 medical and health sciences Mice 0302 clinical medicine Th2 Cells Adjuvants Immunologic Immunity Virology Chlorocebus aethiops medicine Animals 030212 general & internal medicine Horses Japanese encephalitis vaccine Neutralizing antibody Encephalitis Japanese Vero Cells 030304 developmental biology 0303 health sciences Mice Inbred BALB C Reactogenicity biology Japanese Encephalitis Vaccines Animal Immunogenicity Flavivirus Vaccination Inulin Japanese encephalitis Th1 Cells medicine.disease Antibodies Neutralizing Survival Analysis 3. Good health Mice Inbred C57BL Vaccines Inactivated Immunology biology.protein Female Adjuvant medicine.drug |
| Zdroj: | The Journal of General Virology |
| ISSN: | 1465-2099 |
| Popis: | Advax is a polysaccharide-based adjuvant that potently stimulates vaccine immunogenicity without the increased reactogenicity seen with other adjuvants. This study investigated the immunogenicity of a novel Advax-adjuvanted Vero cell culture candidate vaccine against Japanese encephalitis virus (JEV) in mice and horses. The results showed that, in mice, a two-immunization, low-dose (50 ng JEV antigen) regimen with adjuvanted vaccine produced solid neutralizing immunity comparable to that elicited with live ChimeriVax-JE immunization and superior to that elicited with tenfold higher doses of a traditional non-adjuvanted JEV vaccine (JE-VAX; Biken Institute) or a newly approved alum-adjuvanted vaccine (Jespect; Novartis). Mice vaccinated with the Advax-adjuvanted, but not the unadjuvanted vaccine, were protected against live JEV challenge. Equine immunizations against JEV with Advax-formulated vaccine similarly showed enhanced vaccine immunogenicity, confirming that the adjuvant effects of Advax are not restricted to rodent models. Advax-adjuvanted JEV vaccine elicited a balanced T-helper 1 (Th1)/Th2 immune response against JEV with protective levels of cross-neutralizing antibody against other viruses belonging to the JEV serocomplex, including Murray Valley encephalitis virus (MVEV). The adjuvanted JEV vaccine was well tolerated with minimal reactogenicity and no systemic toxicity in immunized animals. The cessation of manufacture of traditional mouse brain-derived unadjuvanted JEV vaccine in Japan has resulted in a JEV vaccine shortage internationally. There is also an ongoing lack of human vaccines against other JEV serocomplex flaviviruses, such as MVEV, making this adjuvanted, cell culture-grown JEV vaccine a promising candidate to address both needs with one vaccine. |
| Databáze: | OpenAIRE |
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