Epitope mapping of an anti-alpha thalassemia/mental retardation syndrome X-linked monoclonal antibody AMab-6
| Autor: | Takuro Nakamura, Shinji Yamada, Hiroyuki Harada, Yoshimi Nakamura, Yoshikazu Furusawa, Masato Fukui, Shunsuke Itai, Saori Handa, Miyuki Yanaka, Yukinari Kato, Mika K. Kaneko, Kayo Hisamatsu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.drug_class Epitope mapping Biophysics PBS phosphate-buffered saline Gene mutation Biology Monoclonal antibody Biochemistry Epitope lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine AMab-6 DAB 3 3-diaminobenzidine tetrahydrochloride medicine Telomerase reverse transcriptase lcsh:QD415-436 mAb monoclonal antibody lcsh:QH301-705.5 ATRX X chromosome ATRX alpha-thalassemia/mental-retardation-syndrome-X-linked ELISA enzyme-linked immunosorbent assay medicine.disease Molecular biology 030104 developmental biology Alpha-thalassemia mental retardation syndrome lcsh:Biology (General) 030217 neurology & neurosurgery Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 15, Iss, Pp 76-80 (2018) |
| ISSN: | 2405-5808 |
| Popis: | The alpha-thalassemia/mental-retardation-syndrome-X-linked (ATRX) gene is located on the q arm of the X chromosome. ATRX gene mutations were first discovered in pancreatic neuroendocrine tumors, and subsequently in other cancer subtypes, including gliomas. Molecular subgrouping of gliomas has been more important than conventional histological classifications. Mutations in the isocitrate dehydrogenase (IDH), telomerase reverse transcriptase (TERT) promoter, and ATRX and the codeletion of chromosomes 1p/19q are used as biomarkers for diagnosing the subtypes of diffuse gliomas. We recently developed a sensitive monoclonal antibody (mAb) AMab-6 against ATRX by immunizing mice with recombinant human ATRX. AMab-6 can help to detect ATRX mutations via Western blotting and immunohistochemical analyses. In this study, we characterized the binding epitope of AMab-6 using enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunohistochemical analysis, and found that Gln2368 of ATRX is critical for AMab-6 binding to ATRX. Our findings could be applied to the production of more functional anti-ATRX mAbs. Highlights • ATRX gene is located on the q arm of the X chromosome. • AMab-6 detected ATRX in Western blot and immunohistochemical analyses sensitively. • Gln2368 of ATRX is critical for AMab-6-binding to ATRX. • A blocking peptide completely neutralized AMab-6 reaction by IHC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|