Evaluation of a nanoflow interface based on the triple-tube coaxial sheath-flow sprayer for capillary electrophoresis-mass spectrometry coupling in metabolomics
| Autor: | Nicolas Drouin, Sabrina Ferré, Víctor González-Ruiz, Serge Rudaz |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Electrospray
Analyte 010402 general chemistry Mass spectrometry CE-ESI-MS 01 natural sciences Biochemistry Capillary electrophoresis–mass spectrometry Analytical Chemistry Capillary electrophoresis Metabolomics Nanotechnology Amino Acids Nanoflow interface chemistry.chemical_classification ddc:615 Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Electrophoresis Capillary Signal Processing Computer-Assisted General Medicine Reference Standards 0104 chemical sciences Volumetric flow rate Amino acid Amino acids Coaxial |
| Zdroj: | Journal of Chromatography. A, Vol. 1641 (2021) P. 461982 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2021.461982 |
| Popis: | The performance of an original CE-MS interface that allows the in-axis positioning of the electrospray with respect to the MS inlet was evaluated. The variations in the geometrical alignment of this configuration in the absence of a nebulizing gas afforded a significant reduction in the sheath-liquid flow rate from 3 µL/min to as low as 300 nL/min. The sheath liquid and BGE were respectively composed of H2O—iPrOH CH3COOH 50:50:1 (v/v/v) and 10% acetic acid (pH 2.2). A significant gain in sensitivity was obtained, and it was correlated to the effective mobility of the analytes. Compounds with low mobility values showed a greater sensitivity gain. Special attention was paid to the detection of proteinogenic amino acids. Linear response functions were obtained from 15 ng/mL to 500 ng/mL. The limits of quantification, as low as 34.3 ng/mL, were improved by a factor of up to six compared to the conventional configuration. The in-axis setup was ultimately applied to the absolute quantification of four important amino acids, alanine, tyrosine, methionine and valine, in standard reference material (NIST plasma). The accuracies ranged from 78 to 113%, thus demonstrating the potential of this configuration for metabolomics. |
| Databáze: | OpenAIRE |
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