Inactivation of Ca2+ Action Potential Channels by the MEK Inhibitor PD98059
| Autor: | Meredith Gould, José Luis Stephano |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
MAPK/ERK pathway Annelida Action Potentials In Vitro Techniques Biology Marine worm Membrane Potentials Human fertilization Nitriles Butadienes medicine Animals Enzyme Inhibitors Protein kinase A Flavonoids Mitogen-Activated Protein Kinase Kinases Sperm-Ovum Interactions Membrane potential Genetics MEK inhibitor Cell Membrane Cell Biology Polyspermy Oocyte Cell biology medicine.anatomical_structure Oocytes Female Calcium Channels |
| Zdroj: | Experimental Cell Research. 260:175-179 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.2000.5014 |
| Popis: | In a previous study involving the inhibition of mitogen-activated protein kinase (MAPK) activation during fertilization of the marine worm Urechis caupo, we found that PD98059, but not U0126, caused multiple sperm penetrations in oocytes (Gould and Stephano, 1999, Dev. Biol. 216, 348–358). Since these oocytes are protected against polyspermy by a positive shift in membrane potential at fertilization (Gould-Somero et al., 1979, J. Cell Biol. 82, 426–440), we investigated the effects of PD98059 on the electrical properties of the oocyte membrane. PD98059, but not U0126, selectively blocked the voltage-dependent Ca2+ channels that participate in the electrical polyspermy block. We also noted previously that PD98059 had more serious effects than U0126 on chromosome behavior during meiosis. This, too, could be explained by the effect on Ca2+ channels, since when U0126-treated eggs were fertilized in low Ca2+ seawater to reduce Ca2+ uptake, similar effects were produced. These results show that PD98059 has side effects unrelated to the inhibition of MAPK activation and underscores the need for caution in interpreting the results of experiments with this widely used MEK inhibitor. |
| Databáze: | OpenAIRE |
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