Properties of promoters cloned randomly from the Saccharomyces cerevisiae genome
| Autor: | G M Santangelo, Kivie Moldave, J Tornow, C S McLaughlin |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Transcription
Genetic Base pair Genetic Vectors Molecular Sequence Data Saccharomyces cerevisiae RNA polymerase II Regulatory Sequences Nucleic Acid DNA Ribosomal Plasmid Transcription (biology) Cloning Molecular DNA Fungal Promoter Regions Genetic Molecular Biology Genetics Reporter gene Base Sequence biology Single-Strand Specific DNA and RNA Endonucleases RNA Promoter Cell Biology Endonucleases biology.organism_classification Molecular biology Gene Expression Regulation biology.protein Research Article |
| Zdroj: | Molecular and Cellular Biology. 8:4217-4224 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.8.10.4217 |
| Popis: | Promoters were isolated at random from the genome of Saccharomyces cerevisiae by using a plasmid that contains a divergently arrayed pair of promoterless reporter genes. A comprehensive library was constructed by inserting random (DNase I-generated) fragments into the intergenic region upstream from the reporter genes. Simple in vivo assays for either reporter gene product (alcohol dehydrogenase or beta-galactosidase) allowed the rapid identification of promoters from among these random fragments. Poly(dA-dT) homopolymer tracts were present in three of five randomly cloned promoters. With two exceptions, each RNA start site detected was 40 to 100 base pairs downstream from a TATA element. All of the randomly cloned promoters were capable of activating reporter gene transcription bidirectionally. Interestingly, one of the promoter fragments originated in a region of the S. cerevisiae rDNA spacer; regulated divergent transcription (presumably by RNA polymerase II) initiated in the same region. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|