Efekat genotipa i inokulacije bakterijama iz roda Bradyrhizobium na morfološke osobine, prinos i sadržaj proteina izabranih sorti soje
| Autor: | Stefan M. Kolašinac, Ilinka Pećinar, Vladimir Miladinovic, Vladan Ugrenovic, Biljana Kiprovski, Zora Dajić-Stevanović, Dragosav Mutavdzic |
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| Rok vydání: | 2021 |
| Předmět: |
Yield (engineering)
Glycine max glycine max Plant Science QH426-470 Biology Bradyrhizobium Protein content 03 medical and health sciences raman spectroscopy Genotype Genetics inoculation soybean 2. Zero hunger 0303 health sciences Inoculation 030305 genetics & heredity food and beverages 15. Life on land protein content yield biology.organism_classification Horticulture varieties Raman spectroscopy Grain yield |
| Zdroj: | Genetika Genetika, Vol 53, Iss 2, Pp 911-925 (2021) Genetika-Belgrade |
| ISSN: | 1820-6069 0534-0012 |
| DOI: | 10.2298/gensr2102911m |
| Popis: | Soybean crop production in Serbia involves seed inoculation by N-fixing bacteria just before sowing time. The main objective of the current work was to assess the impact of the genotype and inoculation on range of morphological and yield traits of soybean (Glycine max L. Merrill), as well as the total protein content. The experiment was conducted on chernozem soil, where soybean was previously grown. The six local varieties were used, where each variety was sown, in three replicates for both inoculated and non-inoculated treatment. The following morphological traits were analysed: the plant height, number of lateral branches, distance to the first pod, number of pods per plant, pods (containing seeds) weight per plant, seed weight per plant, and the total grain yield. The total protein content in seeds was determined by standard analytical method, while subtle differences in qualitative protein composition were assessed using Raman spectroscopy. The total protein content varied from 39.6 to 42.15 %. Performance of inoculation resulted in an increase of the plant height and the distance to the first pod, although not in all tested varieties. The highest and the lowest plant height values were observed for non-inoculated variety Dana (59.23cm) and Sava (80.03cm), respectively. The effect of genotype was much more expressed causing differences in almost all tested characters, except for the total protein content. However, Raman spectroscopy analyses revealed distinct discrimination among surveyed varieties, and differences between inoculated and non-inoculated plants in qualitative composition of seed proteins. Proizvodnja soje u Srbiji uključuje inokulaciju setvenog materijala azotofiksirajućim bakterijama pre setve. Glavni cilj ovog rada je procena uticaja genotipa i inolulacije na morofoške parametre i prinos soje (Glycine max L. Merrill) kao i na sadržaj ukupnih proteina. Eksperiment je sproveden na zemljištu tipa černozem na kojem je prethodno uzgajana soja. Za setvu je korišćeno šest domaćih sorti soje, gde je svaka sorta sejana u tri ponavljanja sa dva tretmana (inokulisano i neinokulisano seme) i praćeni su sledeći morfološki parametri: visina biljke, broj bočnih grana, broj spratova, broj mahuna po biljci, masa mahuna sa semenom po biljci, masa semena po biljci bez mahune i ukupni prinos. Sadržaj ukunih proteina u semenu određen je standardnom analitičkom metodom dok su fine razlike u kvalitativnim osobinama proteina procenjene Ramanovom spektroskopijom. Sadržaj ukunih proteina bio je uniforman i kretao se do 39.6-42.15 %. Inokulacija je dovela do povećanja visine biljke i visina biljke do prve mahune. Najveća i najmanja prosečna visina biljke je uočena kod neinokulisanih sorti Dana (59,23 cm) i Sava (80,03 cm), respektivno. Efekat sorte je bio izraženiji i izazvao je efekat kod svih parametara osim sadržaja ukupnih proteina. Međutim, primena Ramanove spektroskopije je pokazala jasne razlike između ispitivanih sorti u kao i razlike između inokulisanih i neinokulisanih sorti u pogledu kvalitativnog sastava proteina semena. |
| Databáze: | OpenAIRE |
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