On the use of acridinium indicators for the chemiluminescent determination of the total antioxidant capacity of dietary supplements
| Autor: | Karol K Krzymiński, Krzysztof Żamojć, Paweł Błażej Rudnicki-Velasquez, Alexander D. Roshal |
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| Rok vydání: | 2018 |
| Předmět: |
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Antioxidant Luminescence medicine.medical_treatment Dietary supplement Biophysics Succinimides 02 engineering and technology 01 natural sciences Antioxidants law.invention Luminol chemistry.chemical_compound Emission efficiency law medicine Hydrogen peroxide Chemiluminescence Chromatography 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Antioxidant capacity Kinetics chemistry Chemistry (miscellaneous) Dietary Supplements Luminescent Measurements Acridines 0210 nano-technology |
| Zdroj: | Luminescence : the journal of biological and chemical luminescence. 34(5) |
| ISSN: | 1522-7243 |
| Popis: | Acridinium salts, due to their chemiluminogenic properties, have found several applications in biomedical analysis as labels and indicators, where the assessment of emission intensity is used for the end-point detection. This work presents the use of chemiluminescent indicators in the form of selected acridinium esters in order to determine the antioxidant properties of exemplary formulations, namely quercetin, vitamin C and the dietary supplement, Apiextract. The principle of measurements is based on a change in the kinetics of emission decay derived from the acridinium cations in alkaline solutions of hydrogen peroxide in the presence of an antioxidant (the analyte). The proposed system makes a beneficial alternative to related methods, which mostly rely on the assessment of emission efficiency and use the luminometric standard luminol - due to superior parameters of acridinium chemiluminescence, among others - high temporary emission efficiency. The features of the proposed method are manifested by a shorter time period of analysis and lower background signals associated with the environmental influences, as compared to typical approaches. The chromatographic (RP-HPLC) analyses of the substrates and products generated during chemiluminogenic oxidation of acridinium cations under assay conditions are also presented. |
| Databáze: | OpenAIRE |
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