Effect OfN-Acetylcysteine on Carboplatin-Induced Ototoxicity and Nitric Oxide Levels in a Rat Model
| Autor: | M. Akif Kilic, Fatma Inanc Tolun, Ilhami Yildirim, Metin Kilinc, Erdogan Okur |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Hearing Loss Sensorineural medicine.medical_treatment Intraperitoneal injection Antineoplastic Agents Pharmacology Nitric Oxide Carboplatin Nitric oxide Rats Sprague-Dawley Acetylcysteine chemistry.chemical_compound Ototoxicity Evoked Potentials Auditory Brain Stem medicine Animals Saline chemistry.chemical_classification Glutathione Peroxidase business.industry Glutathione peroxidase Free Radical Scavengers medicine.disease Cochlea Rats Disease Models Animal Treatment Outcome Otorhinolaryngology chemistry Spectrophotometry Anesthesia Toxicity business Biomarkers Follow-Up Studies medicine.drug |
| Zdroj: | The Laryngoscope. 117:2183-2186 |
| ISSN: | 1531-4995 0023-852X |
| DOI: | 10.1097/mlg.0b013e31813e6041 |
| Popis: | Objective: The aim of the present study is to investigate the effect of N-acetylcysteine (NAC) given 30 minutes before carboplatin administration on carboplatin-induced ototoxicity and nitric oxide (NO) levels in a rat model. Study Design: Animal study. Methods: Eighteen Sprague-Dawley rats were divided into three groups that each contained six animals. Intraperitoneal injection of physiologic saline was performed in group 1 twice with an interval of 30 minutes. Group 2 was treated with a single bolus administration of carboplatin at a dose of 256 mg/kg 30 minutes after the intraperitoneal injection of physiologic saline. Group 3 was treated with a single bolus administration of carboplatin at a dose of 256 mg/kg 30 minutes after the intraperitoneal injection of NAC at a dose of 400 mg/kg. Pretreatment and posttreatment distortion product otoacoustic emissions (DPOAE) were performed in rats from all groups. Then, the animals were sacrificed on the fourth day, and cochlear tissue NO and glutathione peroxidase (GSH-Px) levels were measured. Results: The comparison of pre- and posttreatment DPOAE responses did not demonstrate any significant changes for groups 1 and 3. Results of group 2 showed a decrease of the DPOAE amplitude. Cochlear NO levels were significantly higher in rats treated with carboplatin than in controls and in those treated with carboplatin plus NAC (P < .05). Cochlear GSH-Px levels were higher in rats treated with carboplatin plus NAC than in those treated with carboplatin, but the difference did not reach statistical significance (P = .079). Conclusions: The present study showed that carboplatin at higher doses induced hearing loss and increased NO levels in the cochlea of rats. NAC appears to have a protective effect against carboplatin-induced ototoxicity, which may be related to its inhibitory effect on NO production. |
| Databáze: | OpenAIRE |
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