Diagnostic characteristics of serological tests for the detection of SARS-CoV-2 cases
| Autor: | D. K. Tverdokhlebova, E V Smeltsova, D M Nikulina, O I Murygina, O V Petrova |
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| Rok vydání: | 2021 |
| Předmět: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Antibodies Viral Roche Diagnostics Sensitivity and Specificity Immunoglobulin G Russia Serology 03 medical and health sciences 0302 clinical medicine Blood serum Humans Medicine Serologic Tests 030212 general & internal medicine 0303 health sciences biology SARS-CoV-2 030306 microbiology business.industry Biochemistry (medical) COVID-19 General Medicine Biological materials body regions Medical Laboratory Technology Immunoglobulin M Immunology biology.protein Antibody business |
| Zdroj: | Russian Clinical Laboratory Diagnostics. 66:210-212 |
| ISSN: | 2412-1320 0869-2084 |
| DOI: | 10.51620/0869-2084-2021-66-4-210-212 |
| Popis: | To study the diagnostic characteristics of test systems for detecting antibodies to SARS-Cov-2. We studied the diagnostic characteristics of two test systems for detecting antibodies to SARS-Cov-2, registered in the Russian Federation. The first test system is a kit for detecting total antibodies to SARS-Cov-2 using immunochemiluminescence analysis on the «Cobas e 411» analyzer («Roche Diagnostics», Germany). The second test system is a kit for detecting IgM and IgG to SARS-Cov-2 («Core Technology Co., Ltd», China) by immunochromatographic analysis. The biological material for the study was blood serum. We assessed: diagnostic sensitivity, diagnostic specificity, and predictive value of positive and negative results. In the test system for detecting total antibodies to SARS-CoV-2, using an IHLA, the diagnostic sensitivity and specificity were 100%; the predictive value of positive and negative results was 100%. In the test system for the detection of IgM and IgG to Sars-CoV-2, using IHA, diagnostic sensitivity for IgM and IgG were 100%; diagnostic specificity for IgM - 60%, for IgG - 72%; predictive value of a positive result for IgM - 60%, IgG - 68,18%; predictive value of negative results for IgM and IgG - 100%. The best diagnostic characteristics were found in the test system for the detection of total antibodies to SARS-Cov-2, which must be taken into account when deciding whether to purchase test systems for the detection of antibodies to SARS-Cov-2. |
| Databáze: | OpenAIRE |
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