Nocardia zapadnayensis sp nov., isolated from soil
| Autor: | Fadime Ozdemir-Kocak, Kamil Isik, Hans-Peter Klenk, Salih Saricaoglu, Gabriele Pötter, Hayrettin Saygin, Kiymet Guven, Cathrin Spröer, Nevzat Sahin, Demet Cetin |
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| Přispěvatelé: | Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Polyphasic Taxonomy Molecular Sequence Data Nocardia Zapadnayensis Nocardia zapadnayensis Diaminopimelic Acid DNA Ribosomal Microbiology Nocardia 16S Rrna Gene Mycolic acid Soil 03 medical and health sciences chemistry.chemical_compound Phylogenetics RNA Ribosomal 16S medicine Molecular Biology Phospholipids Phylogeny Soil Microbiology chemistry.chemical_classification Base Sequence Phylogenetic tree Strain (chemistry) biology Fatty Acids Nocardiosis Nucleic Acid Hybridization Sequence Analysis DNA General Medicine 16S ribosomal RNA medicine.disease biology.organism_classification bacterial infections and mycoses Bacterial Typing Techniques 030104 developmental biology chemistry Microscopy Electron Scanning Polyphasic taxonomy 16S rRNA gene Diaminopimelic acid |
| Popis: | WOS: 000368018200009 PubMed ID: 26547854 A novel Gram-stain positive, rod-shaped, non-motile and mycolic acid containing strain, FMN18(T), isolated from soil, was characterised using a polyphasic approach. The organism showed a combination of morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia and it formed a phyletic line in the Nocardia 16S rRNA gene tree. The cell wall contained meso-diaminopimelic acid (type IV) and whole cell sugars were galactose, glucose, arabinose and ribose. The predominant menaquinone was MK-8(H4 omega-cyclo). The major phospholipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Major fatty acids are C-16:0, 10-methyl C-18:0 (TBSA), C-18:1 cis9 and C-16:1 trans9. These chemotaxonomic traits are in good agreement with those known for representatives of the genus Nocardia. The phylogenetic analysis based on the 16S rRNA gene sequence of strain FMN18(T) showed it to be closely related to Nocardia grenadensis GW5-5797(T) (99.2 %), Nocardia speluncae N2-11(T) (99.1 %), Nocardia jinanensis 04-5195(T) (99.0 %) and Nocardia rhamnosiphila 202GMO(T) (98.3 %). The phylogenetic analysis based on the gyrB gene sequence of strain FMN18(T) showed it to be closely related to N. rhamnosiphila 202GMO(T) (99.0 %), N. grenadensis DSM 45869(T) (96.6 %), N. jinanensis DSM 45048(T) (93.1 %), N. carnea IFM 0237(T) (89.7 %) and N. speluncae DSM 45078(T) (89.1 %). A combination of DNA-DNA hybridization results and phenotypic properties demonstrated that strain FMN18(T) was clearly distinguished from all closely related Nocardia species. It is proposed that the organism be classified as representing a novel species of the genus Nocardia, for which the name Nocardia zapadnayensis (type strain FMN18(T) = DSM 45872(T) = KCTC 29234(T)) is proposed. Ondokuz Mayis University (OMU) [PYO. FEN. 1904.09.009] This research was supported by Ondokuz Mayis University (OMU), project no. PYO. FEN. 1904.09.009. The GenBank accession number for the 16S rRNA gene sequence of N. zapadnayensis FMN18T (=DSM 45872T = KCTC 29234T) is JN896611. The GenBank accession numbers for the gyrB gene sequences for strains Nocardia zapadnayensis FMN18T and N. grenadensis DSM 45869T are JQ040835 and KP313728, respectively. |
| Databáze: | OpenAIRE |
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