Direct interface of chemistry to microbiological systems: membrane inlet mass spectrometry
| Autor: | George Cowie, Katie L. Thomas, David Lloyd, Alan G. Williams, Jonathan D Tammam |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
geography geography.geographical_feature_category Peat Chromatography Bacteria Microorganism Kinetics Analytical chemistry Carbon Dioxide Inlet Silicone rubber Mass spectrometry Microbiology Mass Spectrometry chemistry.chemical_compound Membrane chemistry Cheese Soil water Methane Molecular Biology Soil Microbiology |
| Zdroj: | Journal of Microbiological Methods. 48:289-302 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/s0167-7012(01)00331-1 |
| Popis: | Direct measurement of dissolved gases and low molecular weight volatiles through permeable membranes (e.g. 50-microm-thick silicone rubber), provides an invaluable tool for the investigation of the activities of microorganisms in the laboratory and in their natural environments. Multiple molecular species are monitored at a single point. Fast response times (t(90%)1 min) and long-term stability, (1% week(-1)); high specificity and high sensitivity (e.g. 0.2 microM for O(2),0.5 mM for ethanol), provides a technique that can provide information on the kinetics of processes over many decades (10(0)-10(6)) of minutes. Spatial resolution of1 mm enables 3D mapping of gases in complex ecosystems (sediments, peat, soils, biofilms, foodstuffs). Results with membrane inlet mass spectrometry (MIMS) when used in conjunction with confocal scanning laser microscopy, provides a powerful approach to the analysis of kinetic and spatial aspects of natural environments. Examples discussed are peat cores and cheese. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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