Molecular cloning and expression of bovine interleukin-8
| Autor: | Mohamad A. Morsey, Dale L. Godson, Manuel Campos, Lorne A. Babiuk, Jacek Kowalski, Gerald Gerlach, Yurij Popowych |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Recombinant Fusion Proteins Molecular Sequence Data Biology medicine.disease_cause Microbiology law.invention law Complementary DNA Macrophages Alveolar Gene expression Escherichia coli medicine Animals Humans Amino Acid Sequence RNA Messenger Interleukin 8 Cloning Molecular Mannheimia haemolytica Peptide sequence Cells Cultured Antiserum Base Sequence Sequence Homology Amino Acid Interleukin-8 Sequence Analysis DNA Fusion protein Virology Molecular biology Endotoxins Infectious Diseases Gene Expression Regulation Recombinant DNA Cattle |
| Zdroj: | Microbial Pathogenesis. 20:203-212 |
| ISSN: | 0882-4010 |
| DOI: | 10.1006/mpat.1996.0019 |
| Popis: | Interleukin-8 (IL-8) is a neutrophil and T-lymphocyte chemotactic and activating factor. This cytokine is produced by many cell types including macrophages in response to a variety of microbial and non-microbial agents. In the present study, we determined the nucleotide sequence for bovine IL-8 cDNA. The amino acid sequence encoded by this cDNA shares 76 and 87% homology with the human and swine IL-8 proteins, respectively. Bovine IL-8 cDNA was expressed in Escherichia coli as a beta-galactosidase fusion protein. Western blotting demonstrates that this fusion protein, but not beta-galactosidase cross-reacts with monospecific anti-human IL-8 antiserum. We also studied the induction of IL-8 mRNA synthesis in bovine alveolar macrophages (BAM) stimulated with heat-killed Pasteurella haemolytica. IL-8 mRNA was induced in BAM as early as 1 h and was detectable at high levels 12 h post-stimulation with P. haemolytica. A dose titration of P. haemolytica and E. coli endotoxins showed that a relatively low level of P. haemolytica endotoxin induced high levels of bovine IL-8 mRNA. The significance of these findings in the pathogenesis of bovine pneumonia caused by P. haemolytica is discussed. |
| Databáze: | OpenAIRE |
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