Gonadosomatic index as a confounding variable in fish-based screening assays for the detection of anti-estrogens and nonaromatizable androgens
| Autor: | Kyle S. Roush, Marlo K. Sellin Jeffries |
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| Rok vydání: | 2018 |
| Předmět: |
medicine.drug_class
Health Toxicology and Mutagenesis Cyprinidae Physiology Context (language use) 010501 environmental sciences Biology Endocrine Disruptors 01 natural sciences 03 medical and health sciences Trenbolone Toxicity Tests medicine Environmental Chemistry Animals Gonads 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Reproductive success Fadrozole Reproduction Confounding Estrogen Antagonists Androgen Gonadosomatic Index Androgens Biological Assay Female Trenbolone Acetate Pimephales promelas Water Pollutants Chemical medicine.drug |
| Zdroj: | Environmental toxicology and chemistry. 38(3) |
| ISSN: | 1552-8618 |
| Popis: | The presence of reproductive endocrine-disrupting compounds (REDCs) in the environment poses a potential threat to fish and wildlife, because exposures are capable of altering sexual development, reproductive success, and behavior. Fish-based screening assays are often utilized to screen for the presence of REDCs in surface waters and to assess single chemicals for potential endocrine-disrupting activity. In an effort to improve such screening assays, the goal of the present study was to determine whether the gonadosomatic index (GSI) of female fathead minnows (Pimephales promelas), as assessed via external characteristics, influences their response to REDC exposure. Specifically, we sought to determine whether low-GSI females differed from high-GSI females in their responses to the model anti-estrogen fadrozole and the model androgen 17β-trenbolone, and whether there was a preferable classification in the context of REDC screening. Low-GSI females were more sensitive to fadrozole at the lower concentration of fadrozole (5 µg/L) and to the higher concentration of trenbolone (50 ng/L), whereas high-GSI females were more sensitive at the lower concentration of trenbolone (5 ng/L). The differential response of low- and high-GSI females to REDCs indicates that GSI influences exposure outcome, and should subsequently be taken into consideration in the implementation of screening assays, as failure to utilize fish of the appropriate reproductive status may skew the test results. Environ Toxicol Chem 2019;38:603-615. © 2019 SETAC. |
| Databáze: | OpenAIRE |
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