Flow cytometry of DNA content in human bone marrow: a critical reappraisal
| Autor: | Barthel Barlogie, Wolfgang Göhde, Dennis A. Johnston, Benjamin Drewinko, JL Tekell, Gary M. Dosik |
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| Rok vydání: | 1980 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Immunology Human bone Bone Marrow Cells Sodium Chloride Biology Biochemistry Flow cytometry chemistry.chemical_compound Bone marrow aspirate Bone Marrow Biopsy medicine Humans Saline Reproducibility medicine.diagnostic_test DNA Cell Biology Hematology Pepsin A Blood Cell Count Kinetics Cell Transformation Neoplastic medicine.anatomical_structure chemistry Bone marrow |
| Zdroj: | Blood. 55:734-740 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v55.5.734.734 |
| Popis: | Because cytokinetic studies of the human bone marrow aspirate as a prognostic factor and as a monitor of drug perturbation are frequently inconsistent, we investigated reproducibility of DNA distribution measured by flow cytometry of DNA content in patients with morphologically normal bone marrow. In 15 patients, correlation was noted between DNA distributions simultaneously obtained on right and left iliac crest bone marrow aspirates (r = .588), although considerable variation in individuals was encountered. Much better reproducibility (r = .879) was achieved using bilateral core biopsy of bone marrow in these same patients. In 60 samples, comparison of DNA distribution between bone marrow aspirate and simultaneously obtained biopsy revealed higher relative proportions of S and G2 + M phase cells in biopsies (p less than 0.001), suggesting peripheral blood contamination of aspirate material. Brisk shaking of biopsy specimens in saline expelled a representative sample in the supernatant that could be subjected to simultaneous cytomorphological and cytokinetic analysis. To improve reproducibility of DNA content determinations in normal human bone marrow, bone marrow biopsy should be utilized. |
| Databáze: | OpenAIRE |
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