Cartilage tissue engineering of nasal septal chondrocyte-macroaggregates in human demineralized bone matrix
| Autor: | Juliane Liese, Katharina Stoelzel, A. Haisch, Karym El Sayed, Ulrike Marzahn, Axel Pruss |
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| Rok vydání: | 2012 |
| Předmět: |
Time Factors
Biomedical Engineering Type II collagen Bone Matrix Apoptosis Biocompatible Materials Chondrocyte Biomaterials Chondrocytes Tissue engineering medicine Humans Collagen Type II Cells Cultured Cell Aggregation Cell Proliferation Nasal Septum Transplantation Bone Demineralization Technique Tissue Engineering Caspase 3 Demineralized bone matrix Chemistry Cartilage dBm Cell Differentiation Cell Biology Anatomy Cell aggregation Cell biology medicine.anatomical_structure Type I collagen |
| Zdroj: | Cell and Tissue Banking. 14:255-266 |
| ISSN: | 1573-6814 1389-9333 |
| DOI: | 10.1007/s10561-012-9322-4 |
| Popis: | Tissue Engineering is an important method for generating cartilage tissue with isolated autologous cells and the support of biomaterials. In contrast to various gel-like biomaterials, human demineralized bone matrix (DBM) guarantees some biomechanical stability for an application in biomechanically loaded regions. The present study combined for the first time the method of seeding chondrocyte-macroaggregates in DBM for the purpose of cartilage tissue engineering. After isolating human nasal chondrocytes and creating a three-dimensional macroaggregate arrangement, the DBM was cultivated in vitro with the macroaggregates. The interaction of the cells within the DBM was analyzed with respect to cell differentiation and the inhibitory effects of chondrocyte proliferation. In contrast to chondrocyte-macroaggregates in the cell-DBM constructs, morphologically modified cells expressing type I collagen dominated. The redifferentiation of chondrocytes, characterized by the expression of type II collagen, was only found in low amounts in the cell-DBM constructs. Furthermore, caspase 3, a marker for apoptosis, was detected in the chondrocyte-DBM constructs. In another experimental setting, the vitality of chondrocytes as related to culture time and the amount of DBM was analyzed with the BrdU assay. Higher amounts of DBM tended to result in significantly higher proliferation rates of the cells within the first 48 h. After 96 h, the vitality decreased in a dose-dependent fashion. In conclusion, this study provides the proof of concept of chondrocyte-macroaggregates with DBM as an interesting method for the tissue engineering of cartilage. The as-yet insufficient redifferentiation of the chondrocytes and the sporadic initiation of apoptosis will require further investigations. |
| Databáze: | OpenAIRE |
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