Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
| Autor: | Maria Helena Wohlers Morelli Cardoso, Maria Heloísa Paulino de Moraes, Silvana do Couto Jacob, André Victor Sartori, Bianca Gonçalves Medina |
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| Jazyk: | angličtina |
| Předmět: |
0106 biological sciences
Ochratoxin A Aflatoxin lcsh:TX341-641 01 natural sciences chemistry.chemical_compound 0404 agricultural biotechnology 010608 biotechnology mycotoxins lcsh:Technology (General) cracker wheat-based biscuits Mycotoxin Zearalenone Fumonisin B1 Chromatography 04 agricultural and veterinary sciences 040401 food science chemistry UPLC-MS/MS lcsh:T1-995 Uplc ms ms lcsh:Nutrition. Foods and food supply Food Science Biotechnology Sterigmatocystin |
| Zdroj: | Food Science and Technology, Iss 0 Food Science and Technology, Volume: 39, Issue: 3, Pages: 583-591, Published: 06 JUN 2019 Food Science and Technology v.39 n.3 2019 Food Science and Technology (Campinas) Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA Food Science and Technology, Issue: ahead, Published: 06 JUN 2019 |
| Popis: | An analytical method applying ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was validated for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in crackers. The obtained recoveries (70 to 110%) and relative standard deviations (< 13%) were considered satisfactory. Method limits of quantification ranged from 0.20 µg kg-1 (sterigmatocystin) to 12.27 µg kg-1 (deoxynivalenol). The validated method was then applied for mycotoxin determination in 60 cracker samples (cream crackers and water and salt crackers) obtained from the metropolitan area of the state of Rio de Janeiro, RJ. Deoxynivalenol, zearalenone and fumonisin B1 were found, respectively, in 100, 50 and 28% of the analyzed samples. The maximum permissible limits established by Brazilian legislation for deoxynivalenol and zearalenone were not exceeded in the analyzed samples. |
| Databáze: | OpenAIRE |
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