Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation
| Autor: | Dimitrios Fotakis, Nikos Krigas, Katerina Papanastasi, Dimitris Kyrkas, Eleni Maloupa, Antonis Karydas, Anastasia Badeka, Ioanna S. Kosma, Eleftherios Karapatzak, Paraskevi Yfanti, Ioannis Ganopoulos, Giorgos Patakioutas, Anthimos Zahariadis, Nikos Nikisianis |
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| Rok vydání: | 2021 |
| Předmět: |
Germplasm
ex-situ conservation biodiversity protocols DNA barcoding germplasm phytogenetic resources forest berries Ecology Rosa canina Botany Plant Science Biology biology.organism_classification Ascorbic acid Ex situ conservation Article Horticulture Cutting Phytochemical QK1-989 Ornamental plant Domestication Ecology Evolution Behavior and Systematics |
| Zdroj: | Plants Plants, Vol 10, Iss 2634, p 2634 (2021) Plants; Volume 10; Issue 12; Pages: 2634 |
| ISSN: | 2223-7747 |
| Popis: | Dogroses belong to a taxonomically difficult genus and family and represent important phytogenetic resources associated with high ornamental, pharmaceutical-cosmetic and nutritional values, thus suggesting a potentially high exploitation merit. Triggered by these prospects, wild-growing Rosa canina populations of Greece were selected for investigation and evaluation of their potential for integrated domestication. We collected ripe rosehips from Greek native wild-growing populations (samples from seven genotypes) for phytochemical analysis (total phenolics, total flavonoids, antioxidant activity and vitamin C content), leaf samples for DNA analysis using the ITS2 sequence (nine genotypes) and fresh soft-wood stem cuttings for propagation trials (seven genotypes). After evaluation of these materials, this study reports for the first-time distinct DNA-fingerprinted genotypes from Greece with interesting phytochemical profiles mainly in terms of Vitamic C content (up to 500.22 ± 0.15 mg of ascorbic acid equivalents/100 g of sample) as well as effective asexual propagation protocols for prioritized R. canina genotypes via cuttings. The latter highlights the importance of the levels of external hormone application (2000 ppm of indole-3-butyric acid), the effect of season (highly-effective spring trials) and genotype-specific differences in rooting capacities of the studied genotypes. All inclusive, this study offers new artificially selected material of Greek native R. canina with a consolidated identity and interesting phytochemical profile. These materials are currently under ex-situ conservation for further evaluation and characterization in pilot field studies, thus facilitating its sustainable exploitation for applications in the agro-alimentary, medicinal-cosmetic, and ornamental sectors. |
| Databáze: | OpenAIRE |
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