Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis
| Autor: | Stéfani Karin Martiniano de Almeida, Paulo Sergio Patto dos Santos, Cleber Costa de Martini, Kathlenn Liezbeth Oliveira Silva, Flávia de Rezende Eugênio, Valéria Marçal Felix de Lima, Jéssica Thomé de Andrade |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cellular immunity 040301 veterinary sciences Immunology Lipopolysaccharide Receptors Gene Expression Apoptosis Spleen Biology Nitric Oxide Microbiology Parasite load Peripheral blood mononuclear cell Parasite Load 0403 veterinary science Zoonosis 03 medical and health sciences Dogs Immune system medicine Animals Immunology and Allergy Dog Diseases Flow cytometry Leishmania infantum Visceral leishmaniasis Immunity Cellular General Veterinary Macrophages Nitric oxide Canis familiaris 04 agricultural and veterinary sciences General Medicine medicine.disease Leishmania biology.organism_classification 030104 developmental biology Infectious Diseases medicine.anatomical_structure Case-Control Studies Leukocytes Mononuclear Leishmaniasis Visceral CD14 |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0147-9571 |
| DOI: | 10.1016/j.cimid.2018.01.003 |
| Popis: | Made available in DSpace on 2018-12-11T17:18:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Nitric oxide (NO) is involved in the death of the Leishmania parasite and regulation of apoptosis. We quantified the frequency of cells producing NO and its levels in the peripheral blood mononuclear cells (PBMC), leukocytes from spleen in Visceral Leishmaniasis (VL) symptomatic dogs and correlated NO levels with apoptosis and parasite load in the spleen. The percentage of NO+ cells and CD14+/NO+ was higher in PBMC and spleen cells in infected dogs than in controls. The levels of NO+ and CD14+/NO+ cells was higher in PBMC, but lower spleen of dogs infected than compared to control. Late apoptosis rates increased in PBMC and spleen of infected dogs compared to controls, and the NO levels and apoptosis not showed correlation. There was a positive correlation between the percentage of cells producing NO in the spleen and parasite load. The NO participates in the immune response in the canine VL, but it is not apoptosis inducer. Department of Clinical Care Surgery and Animal Reproduction Laboratory of Cellular Immunology Faculty of Veterinary Medicine of Araçatuba Universidade Estadual Paulista “Julio de Mesquita Filho” FMVA/UNESP, Rua Clovis Pestana, 793, Araçatuba Department of Clinical Care Surgery and Animal Reproduction Laboratory of Cellular Immunology Faculty of Veterinary Medicine of Araçatuba Universidade Estadual Paulista “Julio de Mesquita Filho” FMVA/UNESP, Rua Clovis Pestana, 793, Araçatuba |
| Databáze: | OpenAIRE |
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