Tityus serrulatus scorpion venom improves survival and lung inflammation in lethal sepsis induced by CLP in mice

Autor: Flávia R.F. Nascimento, Eder Magalhães Silva Fialho, Valéria M. Borges, Fábio H. Kwasniewski, Rosane N. M. Guerra, Márcia C.G. Maciel
Rok vydání: 2014
Předmět:
Zdroj: Toxicon : official journal of the International Society on Toxinology. 89
ISSN: 1879-3150
Popis: Tityus serrulatus venom (Tsv) modifies the behavior of immune cells and induces the production of inflammatory and anti-inflammatory cytokines; such action may interfere with physiological or pathological states. Because sepsis is characterized as an inflammatory disorder, the aim of present study was to investigate the effect of a non-lethal dose of Tsv in mice submitted to a polymicrobial infection by cecal ligation and puncture (CLP) model. The parameters evaluated were survival index, cellularity on lymphoid organs, peritoneal cavity and brochoalveolar space, production of IL-10, IL-12, IL-6, TNF-α, IFN-γ and MCP-1, pulmonary inflammation and oxidative burst. The results demonstrated that in sharp contrast to CLP group in which sepsis was lethal in a 24 h period all mice pretreated with Tsv survived even 60 h after CLP. Lung inflammation, another hallmark of CLP group, was also dramatically down regulated in Tsv/CLP group. Despite pretreatment with Tsv did not reduce the inflammatory serum cytokines when compared to CLP group; there was an increase in IL-10. In conclusion, subcutaneous Tsv administration 6 h before CLP was able to control the harmful effects of sepsis (lethality and lung inflammation). We suggest that both systemic IL-10 and oxidative burst are involved in this effect.
Databáze: OpenAIRE
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