Development of a novel and rapid phenotype-based screening method to assess rice seedling growth
| Autor: | Mary Kay Harper, Christine Jewel C. Uy, Chananchida Sang-Aram, Dirk Inzé, Stephen Depuydt, Deborah Botterman, Lena Vlaminck, Godelieve Gheysen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Automated computer analysis ROOT-GROWTH Oryza sativa Plant Science TOXICITY ASSESSMENT Biology lcsh:Plant culture 01 natural sciences 03 medical and health sciences RIVA Genetics Arabidopsis thaliana Bioassay lcsh:SB1-1110 Plant breeding Seedling growth lcsh:QH301-705.5 CROP IMPROVEMENT Plength ELONGATION business.industry Abiotic stress ORYZA-SATIVA L Methodology food and beverages Biology and Life Sciences biology.organism_classification MARINE SPONGES Biotechnology 030104 developmental biology lcsh:Biology (General) Seedling ALKALOIDS Marine natural products Shoot Phytotoxicity Phenotype-based screening business ALUMINUM TOLERANCE INHIBITORS 010606 plant biology & botany GERMINATION |
| Zdroj: | PLANT METHODS Plant Methods, Vol 16, Iss 1, Pp 1-19 (2020) Plant Methods |
| ISSN: | 1746-4811 |
| Popis: | Background Rice (Oryza sativa) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for Arabidopsis thaliana. Here, we present a phenotype-based screening method to study shoot-related parameters of rice seedlings via an automated computer analysis. Results The phenotype-based screening method was validated by testing several compounds in pharmacological experiments that interfered with hormone homeostasis, confirming that the assay was consistent with regard to the anticipated plant growth regulation and revealing the robustness of the set-up in terms of reproducibility. Moreover, abiotic stress tests using NaCl and DCMU, an electron transport blocker during the light dependent reactions of photosynthesis, confirmed the validity of the new method for a wide range of applications. Next, this method was used to screen the impact of semi-purified fractions of marine invertebrates on the initial stages of rice seedling growth. Certain fractions clearly stimulated growth, whereas others inhibited it, especially in the root, illustrating the possible applications of this novel, robust, and fast phenotype-based screening method for rice. Conclusions The validated phenotype-based and cost-efficient screening method allows a quick and proper analysis of shoot growth and requires only small volumes of compounds and media. As a result, this method could potentially be used for a whole range of applications, ranging from discovery of novel biostimulants, plant growth regulators, and plant growth-promoting bacteria to analysis of CRISPR knockouts, molecular plant breeding, genome-wide association, and phytotoxicity studies. The assay system described here can contribute to a better understanding of plant development in general. |
| Databáze: | OpenAIRE |
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