Normative data for flow cytometry immunophenotyping of benign lymph nodes sampled by surgical biopsy
| Autor: | Susan K. Atwater, Gregory D. Scott, Dita Gratzinger |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Male Pathology Biopsy T-Lymphocytes Antigens CD7 CD8-Positive T-Lymphocytes 0302 clinical medicine Immunophenotyping Reference Values hemic and lymphatic diseases T-cell lymphoma informatics Child Lymph node Aged 80 and over B-Lymphocytes medicine.diagnostic_test General Medicine Middle Aged lymph node immunophenotype medicine.anatomical_structure 030220 oncology & carcinogenesis Child Preschool Female Neprilysin Lymph benign Adult medicine.medical_specialty Adolescent Antigens CD19 Short Report CD5 Antigens Pathology and Forensic Medicine 03 medical and health sciences Young Adult medicine Humans Aged business.industry flow cytometry Infant medicine.disease Lymphoma 030104 developmental biology Concomitant Lymph Nodes CD5 business Biomarkers Follow-Up Studies |
| Zdroj: | Journal of Clinical Pathology |
| ISSN: | 1472-4146 |
| Popis: | AimsTo create clinically relevant normative flow cytometry data for understudied benign lymph nodes and characterise outliers.MethodsClinical, histological and flow cytometry data were collected and distributions summarised for 380 benign lymph node excisional biopsies. Outliers for kappa:lambda light chain ratio, CD10:CD19 coexpression, CD5:CD19 coexpression, CD4:CD8 ratios and CD7 loss were summarised for histological pattern, concomitant diseases and follow-up course.ResultsWe generated the largest data set of benign lymph node immunophenotypes by an order of magnitude. B and T cell antigen outliers often had background immunosuppression or inflammatory disease but did not subsequently develop lymphoma.ConclusionsDiagnostic immunophenotyping data from benign lymph nodes provide normative ranges for clinical use. Outliers raising suspicion for B or T cell lymphoma are not infrequent (26% of benign lymph nodes). Caution is indicated when interpreting outliers in the absence of excisional biopsy or clinical history, particularly in patients with concomitant immunosuppression or inflammatory disease. |
| Databáze: | OpenAIRE |
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