The Fun30 Chromatin Remodeler Fft3 Controls Nuclear Organization and Chromatin Structure of Insulators and Subtelomeres in Fission Yeast
| Autor: | Jean-Paul Javerzat, Olga Khorosjutina, Babett Steglich, Karl Ekwall, Agata Smialowska, Annelie Strålfors, Jenna Persson |
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| Přispěvatelé: | Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Transcription Genetic Chromosomal Proteins Non-Histone [SDV]Life Sciences [q-bio] RNA Transfer Gene Expression Regulation Fungal Nuclear protein Genetics (clinical) Genetics Nuclear Proteins Telomere Subtelomere Chromatin Cell biology Nucleosomes MESH: Schizosaccharomyces MESH: Terminal Repeat Sequences Insulator Elements MESH: Membrane Proteins MESH: Gene Expression Regulation Fungal Bivalent chromatin Research Article MESH: Cell Nucleus lcsh:QH426-470 Biology Chromatin remodeling MESH: Chromatin MESH: Chromosomal Proteins Non-Histone MESH: Nucleosomes Schizosaccharomyces Nucleosome Scaffold/matrix attachment region Molecular Biology Ecology Evolution Behavior and Systematics ChIA-PET Cell Nucleus MESH: Transcription Genetic Terminal Repeat Sequences MESH: Chromatin Assembly and Disassembly Membrane Proteins MESH: Schizosaccharomyces pombe Proteins MESH: Insulator Elements Chromatin Assembly and Disassembly MESH: RNA Transfer lcsh:Genetics Schizosaccharomyces pombe Proteins MESH: Telomere MESH: Nuclear Proteins |
| Zdroj: | PLoS Genetics PLoS Genetics, Public Library of Science, 2015, 11 (3), pp.e1005101. ⟨10.1371/journal.pgen.1005101⟩ PLoS Genetics, Vol 11, Iss 3, p e1005101 (2015) |
| ISSN: | 1553-7390 1553-7404 |
| DOI: | 10.1371/journal.pgen.1005101⟩ |
| Popis: | In eukaryotic cells, local chromatin structure and chromatin organization in the nucleus both influence transcriptional regulation. At the local level, the Fun30 chromatin remodeler Fft3 is essential for maintaining proper chromatin structure at centromeres and subtelomeres in fission yeast. Using genome-wide mapping and live cell imaging, we show that this role is linked to controlling nuclear organization of its targets. In fft3∆ cells, subtelomeres lose their association with the LEM domain protein Man1 at the nuclear periphery and move to the interior of the nucleus. Furthermore, genes in these domains are upregulated and active chromatin marks increase. Fft3 is also enriched at retrotransposon-derived long terminal repeat (LTR) elements and at tRNA genes. In cells lacking Fft3, these sites lose their peripheral positioning and show reduced nucleosome occupancy. We propose that Fft3 has a global role in mediating association between specific chromatin domains and the nuclear envelope. Author Summary In the genome of eukaryotic cells, domains of active and repressive chromatin alternate along the chromosome arms. Insulator elements are necessary to shield these different environments from each other. In the fission yeast Schizosaccharomyces pombe, the chromatin remodeler Fft3 is required to maintain the repressed subtelomeric chromatin. Here we show that Fft3 maintains nucleosome structure of insulator elements at the subtelomeric borders. We also observe that subtelomeres and insulator elements move away from the nuclear envelope in cells lacking Fft3. The nuclear periphery is known to harbor repressive chromatin in many eukaryotes and has been implied in insulator function. Our results suggest that chromatin remodeling through Fft3 is required to maintain proper chromatin structure and nuclear organization of insulator elements. |
| Databáze: | OpenAIRE |
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