Intracellular σ 1 receptor modulates phospholipase C and protein kinase C activities in the brainstem
| Autor: | E. E. Baulieu, François P. Monnet, Marie-Pierre Morin-Surun, T. Collin, Monique Denavit-Saubié |
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| Přispěvatelé: | PERIGNON, Alain, Institut de Neurobiologie Alfred Fessard (INAF), Centre National de la Recherche Scientifique (CNRS), Neurobiologie génétique et intégrative (NGI), Laboratoire de Neurobiologie, Université de Picardie Jules Verne (UPJV), Stéroides et système nerveux : physiopathologie moléculaire et clinique, Université Paris-Sud - Paris 11 (UP11)-IFR93-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 1999 |
| Předmět: |
Male
Cytoplasm Pentazocine MESH: Pentazocine Action Potentials Ligands Tropomyosin receptor kinase C Phenazocine MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Ligands MESH: Animals 5-HT5A receptor Virulence Factors Bordetella Protein Kinase C MESH: Action Potentials Protease-activated receptor 2 Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Biological Sciences MESH: Receptors sigma Isoenzymes MESH: Phenazocine Biochemistry MESH: Isoenzymes MESH: Respiratory Mechanics [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Type C Phospholipases Antipsychotic Agents MESH: GTP-Binding Proteins Guinea Pigs In Vitro Techniques MESH: Virulence Factors Bordetella MESH: Pertussis Toxin Biology MESH: Guinea Pigs GTP-Binding Proteins Protein Kinase C beta Enzyme-linked receptor Animals Receptors sigma [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] G protein-coupled receptor G protein-coupled receptor kinase MESH: Cytoplasm Liver receptor homolog-1 Cell Membrane MESH: Protein Kinase C MESH: Male MESH: Haloperidol Neuron-derived orphan receptor 1 Pertussis Toxin Type C Phospholipases MESH: Brain Stem Respiratory Mechanics Haloperidol MESH: Antipsychotic Agents Brain Stem MESH: Cell Membrane |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 1999, 96 (14), pp.8196-9 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.96.14.8196 |
| Popis: | Most physiological effects of σ 1 receptor ligands are sensitive to pertussis toxin, suggesting a coupling with cell membrane-bound G proteins. However, the cloning of the σ 1 receptor has allowed the identification of an intracellular protein anchored on the endoplasmic reticulum. Here, we show, using the isolated adult guinea pig brainstem preparation, that activation of the σ 1 receptor results in its translocation from the cytosol to the vicinity of the cell membrane and induces a robust and rapid decrease in hypoglossal activity, which is mediated by phospholipase C. The subsequent activation of protein kinase C β 1 and β 2 isoforms and the phosphorylation of a protein of the same molecular weight as the cloned σ 1 receptor lead to a desensitization of the σ 1 motor response. Our results indicate that the intracellular σ 1 receptor regulates several components implicated in plasma membrane-bound signal transduction. This might be an example of a mechanism by which an intracellular receptor modulates metabotropic responses. |
| Databáze: | OpenAIRE |
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