Mechanical stress compromises multicomponent efflux complexes in bacteria
| Autor: | Melanie F. Roberts, Bing Fu, Xuanhao Sun, Abhishek Srivastava, Xianwen Mao, Christine E. Harper, Won Jung, Lauren A. Genova, Chung-Yuen Hui, Christopher J. Hernandez, Peng Chen, Łukasz Krzemiński, Lucy M. Wang, Ace George Santiago, Yu-Chern Wong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cell division
Cell Motility Bacterial growth Bacterial cell structure Diffusion 03 medical and health sciences 0302 clinical medicine medicine Escherichia coli 030304 developmental biology 0303 health sciences Multidisciplinary biology Chemistry Escherichia coli Proteins 030302 biochemistry & molecular biology Cell Membrane Biofilm Membrane Proteins Membrane Transport Proteins biology.organism_classification Single Molecule Imaging Biomechanical Phenomena medicine.anatomical_structure Physical Sciences Biophysics Efflux Stress Mechanical Cell envelope Bacterial outer membrane 030217 neurology & neurosurgery Bacteria |
| Zdroj: | Proc Natl Acad Sci U S A |
| Popis: | Physical forces have long been recognized for their effects on the growth, morphology, locomotion, and survival of eukaryotic organisms1. Recently, mechanical forces have been shown to regulate processes in bacteria, including cell division2, motility3, virulence4, biofilm initiation5,6, and cell shape7,8, although it remains unclear how mechanical forces in the cell envelope lead to changes in molecular processes. In Gram-negative bacteria, multicomponent protein complexes that form rigid links across the cell envelope directly experience physical forces and mechanical stresses applied to the cell. Here we manipulate tensile and shear mechanical stress in the bacterial cell envelope and use single-molecule tracking to show that shear (but not tensile) stress within the cell envelope promotes disassembly of the tripartite efflux complex CusCBA, a system used by E. coli to resist copper and silver toxicity, thereby making bacteria more susceptible to metal toxicity. These findings provide the first demonstration that mechanical forces, such as those generated during colony overcrowding or bacterial motility through submicron pores, can inhibit the contact and function of multicomponent complexes in bacteria. As multicomponent, trans-envelope efflux complexes in bacteria are involved in many processes including antibiotic resistance9, cell division10, and translocation of outer membrane components11, our findings suggest that the mechanical environment may regulate multiple processes required for bacterial growth and survival. |
| Databáze: | OpenAIRE |
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