EDTA-Anticoagulated Whole Blood for SARS-CoV-2 Antibody Testing by Electrochemiluminescence Immunoassay (ECLIA) and Enzyme-Linked Immunosorbent Assay (ELISA)
| Autor: | Pietro Vernazza, Sarah Thiel, Francesca Ferrara, Dorothea Hillmann, Susanna Bigler, Lorenz Risch, Konrad Egli, Martin Risch, Nadja Wohlwend, Michael Ritzler, Matthias Paprotny, Marc Kovac, Thomas Bodmer, Christian R Kahlert, Thomas Lung, Mauro Imperiali, Myriam Weber, Kirsten Grossmann, Philipp Kohler, Sonja Heer, Harald Renz, Yacir Salimi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology Clinical Biochemistry specificity Diagnostic accuracy Hematocrit Article Lateral flow test 03 medical and health sciences Electrochemiluminescence Medicine antibodies Whole blood lcsh:R5-920 Chromatography medicine.diagnostic_test biology business.industry SARS-CoV-2 fungi whole blood Area under the curve COVID-19 sensitivity 030104 developmental biology biology.protein Antibody business lcsh:Medicine (General) serum preanalytics |
| Zdroj: | Diagnostics, Vol 10, Iss 593, p 593 (2020) Diagnostics Volume 10 Issue 8 |
| ISSN: | 2075-4418 |
| Popis: | While lateral flow test formats can be utilized with whole blood and low sample volumes, their diagnostic characteristics are inferior to immunoassays based on chemiluminescence immunoassay (CLIA) or enzyme-linked immunosorbent assay (ELISA) technology. CLIAs and ELISAs can be automated to a high degree but commonly require larger serum or plasma volumes for sample processing. We addressed the suitability of EDTA-anticoagulated whole blood as an alternative sample material for antibody testing against SARS-CoV-2 by electro-CLIA (ECLIA Roche, Rotkreuz, Switzerland) and ELISA (IgG and IgA Euroimmun, Germany). Simultaneously drawn venous serum and EDTA-anticoagulated whole blood samples from 223 individuals were included. Correction of the whole blood results for hematocrit led to a good agreement with the serum results for weakly to moderately positive antibody signals. In receiver-operating characteristic curve analysis, all three assays displayed comparable diagnostic accuracy (area under the curve (AUC)) using corrected whole blood and serum (AUCs: 0.97 for ECLIA and IgG ELISA 0.84 for IgA ELISA). In conclusion, our results suggest that the investigated assays can reliably detect antibodies against SARS-CoV-2 in hemolyzed whole blood anticoagulated with EDTA. Correction of these results for hematocrit is suggested. This study demonstrates that the automated processing of whole blood for identification of SARS-CoV-2 antibodies with common ECLIA and ELISA methods is accurate and feasible. |
| Databáze: | OpenAIRE |
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