Effects of linalool and eugenol on the survival of Leishmania ( L .) infantum chagasi within macrophages
| Autor: | Daniela S. Alviano, Mauricio M. Oliveira, Angela H. Lopes, Fernando L. Dutra, Reinaldo Sousa Dos Santos, Wagner Seixas da Silva, Danielle P. Vieira |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Insecticides Acyclic Monoterpenes Veterinary (miscellaneous) Biology Microbiology 03 medical and health sciences chemistry.chemical_compound Linalool Eugenol parasitic diseases medicine Animals Leishmania infantum Trypanosoma cruzi Infectivity Mice Inbred BALB C 030102 biochemistry & molecular biology biology.organism_classification Leishmania medicine.disease Pentavalent antimonial 030104 developmental biology Infectious Diseases Visceral leishmaniasis chemistry Insect Science Immunology Macrophages Peritoneal Monoterpenes Antimonial Parasitology |
| Zdroj: | Acta Tropica. 164:69-76 |
| ISSN: | 0001-706X |
| Popis: | The most commonly used drugs against visceral leishmaniasis are based on pentavalent antimonial compounds, which have played a fundamental role in therapy for over 70 years. However, the treatment is painful and has severe toxic side effects that can be fatal. Antimonial resistance is spreading and reaching alarming proportions. Linalool and eugenol have been shown to kill Leishmania (L.) amazonensis and Trypanosoma cruzi at low doses. In the present study, we demonstrate the effects of linalool and eugenol, components of essential oils, on Leishmania (L.) infantum chagasi, one of the causative agents of visceral leishmaniasis. We compared the effects of those compounds to the effects of glucantime, a positive control. In L. infantum chagasi killing assays, the LD50 for eugenol was 220 μg/ml, and that for linalool was 550 μg/ml. L. infantum chagasi was added to cultures of peritoneal mouse macrophages for four hours prior to drug treatment. Eugenol and linalool significantly decreased the number of parasites within the macrophages. Eugenol and linalool enhanced the activities of the L. infantum chagasi protein kinases PKA and PKC. Linalool also decreased L. infantum chagasi oxygen consumption. In conclusion, both linalool and eugenol promoted a decrease in the proliferation and viability of L. infantum chagasi. These effects were more pronounced during the interaction between the parasites and peritoneal mouse macrophages. |
| Databáze: | OpenAIRE |
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