Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae
| Autor: | Radclyffe L. Roberts, Gerald R. Fink, Hans-Ulrich Mosch |
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| Rok vydání: | 1996 |
| Předmět: |
MAPK/ERK pathway
Saccharomyces cerevisiae Proteins Genotype Cell Cycle Proteins macromolecular substances Saccharomyces cerevisiae CDC42 Protein Serine-Threonine Kinases GTP Phosphohydrolases Fungal Proteins Pseudohyphal growth GTP-Binding Proteins Animals Protein kinase A Mammals Recombination Genetic cdc42 GTP-Binding Protein Saccharomyces cerevisiae Multidisciplinary biology MAP kinase kinase kinase Kinase Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases beta-Galactosidase Molecular biology Recombinant Proteins Cell biology Mitogen-activated protein kinase Calcium-Calmodulin-Dependent Protein Kinases ras Proteins biology.protein Signal transduction Plasmids Signal Transduction Transcription Factors Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:5352-5356 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | RAS2val19, a dominant activated form of Saccharomyces cerevisiae Ras2, stimulates both filamentous growth and expression of a transcriptional reporter FG(TyA)::lacZ but does not induce the mating pathway reporter FUS1::lacZ. This induction depends upon elements of the conserved mitogen-activated protein kinase (MAPK) pathway that is required for both filamentous growth and mating, two distinct morphogenetic events. Full induction requires Ste20 (homolog of mammalian p65PAK protein kinases), Ste11 [an MEK kinase (MEKK) or MAPK kinase (MEK) kinase], Ste7 (MEK or MAPK kinase), and the transcription factor Ste12. Moreover, the Rho family protein Cdc42, a conserved morphogenetic G protein, is also a potent regulator of filamentous growth and FG(TyA)::lacZ expression in S. cerevisiae. Stimulation of both filamentous growth and FG(TyA)::lacZ by Cdc42 depends upon Ste20. In addition, dominant negative CDC42Ala118 blocks RAS2val19 activation, placing Cdc42 downstream of Ras2. Our results suggest that filamentous growth in budding yeast is regulated by an evolutionarily conserved signaling pathway that controls cell morphology. |
| Databáze: | OpenAIRE |
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