Regulatory T cells in the actinic cheilitis
Autor: | Edgard Franco de Mello, Thaís Helena Gasparoto, João Santana da Silva, Maura Rosane Valério Ikoma, Maria Renata Sales Nogueira Costa, Ana Paula Campanelli, Tatiana Salles de Souza Malaspina, Gustavo Pompermaier Garlet, Karen A. Cavassani, José Humberto Damante |
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Rok vydání: | 2014 |
Předmět: |
Adult
CD4-Positive T-Lymphocytes Cancer Research medicine.medical_treatment Lymphocyte proliferation Lymphocyte Activation T-Lymphocytes Regulatory Pathology and Forensic Medicine Interleukin 21 Interferon-gamma Immune system Transforming Growth Factor beta medicine Tumor Microenvironment Humans IL-2 receptor Lymphocyte Count Aged Cell Proliferation Aged 80 and over business.industry Actinic cheilitis Interleukin-2 Receptor alpha Subunit Interleukin Middle Aged medicine.disease CÉLULAS Interleukin-10 Interleukin 10 Cytokine Phenotype Otorhinolaryngology Cheilitis Case-Control Studies Immunology CD4 Antigens Lip Neoplasms Leukocytes Mononuclear Periodontics Oral Surgery Inflammation Mediators business Precancerous Conditions |
Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
Popis: | Background Actinic cheilitis (AC) is an oral potentially malignant lesion which is the counterpart of actinic keratosis of the skin and has potential to develop into squamous cell carcinoma. Regulatory T cells (Tregs) have a critical role in modulating the antitumor immune responses. The presence of regulatory T cells in potentially malignant lesions has not been described. We chose investigate the involvement of regulatory T cells in potentially malignant lesions. Methods The frequency, phenotype, and activity of CD4+CD25+ T cells isolated from blood and lesion of AC patients were analyzed by flow cytometry. Cytokines were quantified by ELISA. Data were compared with samples from healthy subjects. Results The frequency and suppressor activity of circulating CD4+CD25+ T cells was similar in AC patients and control subjects. However, the frequencies of IL-10-positive Tregs were higher in AC patients, and these cells inhibited interferon-gamma (IFN-γ) and increased interleukin (IL)-10 productions in co-cultures. Furthermore, CD4+CD25+ T cells accumulate in AC lesions. Lesions-derived regulatory T cells suppressed lymphocyte proliferation and pro-inflammatory cytokine production. Moreover, high levels of IL-10 and transforming growth factor-β (TGF-β), and low IFN-γ were detected in the potentially malignant lesions. Conclusion Therefore, our data show that Tregs accumulate in AC lesions, and these cells could be suppressing immune responses in a potentially malignant microenvironment. |
Databáze: | OpenAIRE |
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