Sex-dependent role of vesicular glutamate transporter 3 in stress-regulation and related anxiety phenotype during the early postnatal period

Autor:	Diána Balázsfi, Dóra Zelena, Anna Fodor, József Haller, Sándor Zsebők, Lívia Farkas, Péter Csikota
Rok vydání:	2016
Předmět:	Male 0301 basic medicine Hypothalamo-Hypophyseal System medicine.medical_specialty Lipopolysaccharide Amino Acid Transport Systems Acidic Physiology Glutamic Acid Pituitary-Adrenal System Anxiety Mice 03 medical and health sciences Behavioral Neuroscience chemistry.chemical_compound Glutamatergic 0302 clinical medicine Corticosterone Internal medicine medicine Animals Mice Knockout Neurons Maternal deprivation Endocrine and Autonomic Systems Maternal Deprivation Wild type Glutamate receptor Glutamic acid Psychiatry and Mental health 030104 developmental biology Neuropsychology and Physiological Psychology Endocrinology Animals Newborn chemistry Female Vocalization Animal medicine.symptom Psychology 030217 neurology & neurosurgery
Zdroj:	Stress. 19:434-438
ISSN:	1607-8888 1025-3890
Popis:	Stress and related disorders are in the focus of interest and glutamate is one of the most important neurotransmitters that can affect these processes. Glutamatergic neurons are characterized by vesicular glutamate transporters (VGluT1-3) among which vGluT3 is unique contributing to the non-canonical, neuromodulatory effect of glutamate. We aimed to study the role of vGluT3 in stress axis regulation and related anxiety during the early postnatal period using knockout (KO) mice with special focus on sex differences. Anxiety was explored on postnatal day (PND) 7-8 by maternal separation-induced ultrasonic vocalization (USV). Stress-hormone levels were detected 60 min after intraperitoneal lipopolysaccharide (LPS) injection 7 days later. Both genotypes gained weight, but on PND 14-15 KO mice pups had smaller body weight compared to wild type (WT). vGluT3 KO mice reacted to an immune stressor with enhanced adrenocorticotropin (ACTH) and corticosterone secretion compared to WT. Although there was a tendency for enhanced anxiety measured by more emitted USV, this did not reach the level of significance. The only sex-related effect was the enhanced corticosterone reactivity in male pups. For the HPA axis regulation in neonates vGluT3 expression seems to be dispensable under basal conditions, but is required for optimal response to immune stressors, most probably through an interaction with other neurotransmitters. Disturbance of the fine balance between these systems may result in a borderline enhanced anxiety-like behavior in vGluT3 KO pups.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b270a7dcc2a6526829589b0579bd59d5 https://doi.org/10.1080/10253890.2016.1203413 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.