Comparative Biochemical Analysis of Cellulosomes Isolated from Clostridium clariflavum DSM 19732 and Clostridium thermocellum ATCC 27405 Grown on Plant Biomass
| Autor: | Mitsuru Haruki, Takaaki Kokuzawa, Katsuaki Hirano, Nobutaka Hirano, Masahiro Kurosaki, Suguru Shinoda, Hatsumi Takano, Kenji Ueda |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Bioengineering Dockerin Cellulosomes 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Cellulosome Clostridium thermocellum Clostridium 010608 biotechnology Biomass Molecular Biology Cell Proliferation biology 010405 organic chemistry Chemistry food and beverages Oryza General Medicine biology.organism_classification Xylan 0104 chemical sciences Xylanase Anaerobic bacteria Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 187(3) |
| ISSN: | 1559-0291 |
| Popis: | The cellulosome is a supramolecular multienzyme complex formed via species-specific interactions between the cohesin modules of scaffoldin proteins and the dockerin modules of a wide variety of polysaccharide-degrading enzymes. Here, we report a comparative analysis of cellulosomes prepared from the thermophilic anaerobic bacteria Clostridium (Ruminiclostridium) clariflavum DSM 19732 and Clostridium (Ruminiclostridium) thermocellum ATCC 27405 grown on delignified rice straw. The results indicate that the isolated C. clariflavum cellulosome exhibits lower activity for insoluble cellulosic substrates and higher activity for hemicellulosic substrates, especially for xylan, compared to the isolated C. thermocellum cellulosome. The C. clariflavum cellulosome was separated into large and small complexes by size exclusion chromatography, and the high xylanase activity of the intact complex is mainly attributed to the small complex. Furthermore, both C. clariflavum and C. thermocellum cellulosomes efficiently converted delignified rice straw into soluble sugars with different compositions, whereas a mixture of these cellulosomes exhibited essentially no synergy for the saccharification of delignified rice straw. This is the first study to report that isolated C. clariflavum cellulosomes exhibit greater xylanase activity than isolated C. thermocellum cellulosomes. We also report the effect of a combination of intact cellulosome complexes isolated from different species on the saccharification of plant biomass. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink