Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats
| Autor: | Ricardo Simoes, Manuel Simões, Luiz Fernando Portugal Fuchs, Carla Cristina Maganhin, Edmund Chada Baracat, José Maria Soares, Gisela Rodrigues da Silva Sasso |
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| Rok vydání: | 2014 |
| Předmět: |
endocrine system
medicine.medical_specialty Hormone Replacement Therapy medicine.medical_treatment Pinealectomy Ovary Pineal Gland Melatonin Pineal gland Aromatase Internal medicine Gene expression PINEALECTOMIA ANIMAL medicine Animals Cholesterol Side-Chain Cleavage Enzyme RNA Messenger Rats Wistar Progesterone biology Cholesterol side-chain cleavage enzyme Theca interna Steroid 17-alpha-Hydroxylase Obstetrics and Gynecology Estrogens Rats medicine.anatomical_structure Endocrinology Reproductive Medicine Steroid Hydroxylases biology.protein Female medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2014.04.006 |
| Popis: | Objective To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. Design Experimental research. Setting University research laboratory. Animal(s) Thirty female adult rats. Intervention(s) Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 μg/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. Main Outcome Measure(s) Biologic molecular study followed by immunohistochemical analysis. Result(s) The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. Conclusion(s) Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. |
| Databáze: | OpenAIRE |
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