Gga-miR-21 inhibits chicken pre-adipocyte proliferation in part by down-regulating Kruppel-like factor 5
| Autor: | Hui Li, Ning Wang, Yuxiang Wang, Shupei Qiao, Min Cheng, Weishi Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Kruppel-Like Transcription Factors Endogeny Avian Proteins 03 medical and health sciences Krüppel RNA interference Gene expression Adipocytes Animals Cell Proliferation Messenger RNA Gene knockdown Base Sequence biology Cell growth Chemistry General Medicine Cell biology Proliferating cell nuclear antigen MicroRNAs 030104 developmental biology Gene Expression Regulation biology.protein Animal Science and Zoology Chickens |
| Zdroj: | Poultry Science. 96:200-210 |
| ISSN: | 0032-5791 |
| Popis: | Gga-miR-21 is abundantly expressed in chicken pre-adipocytes, but its role is unclear. The present study investigated the role of gga-miR-21 in chicken pre-adipocyte proliferation. Cell proliferation assay and gene expression analysis of proliferating cell nuclear antigen (PCNA) showed that the gga-miR-21 mimic inhibited pre-adipocyte proliferation. In contrast, the gga-miR-21 inhibitor enhanced pre-adipocyte proliferation. The subsequent investigation identified Kruppel-like factor 5 (KLF5) mRNA as a target of gga-miR-21. The gga-miR-21 mimic inhibited KLF5 3'UTR reporter activity and decreased endogenous KLF5 expression in primary pre-adipocytes. KLF5 knockdown using RNAi had a similar effect to that of the gga-miR-21 mimic on cell proliferation. The promoting effect of the gga-miR-21 inhibitor on pre-adipocyte proliferation was partially attenuated by KLF5 knockdown. Taken together, our results demonstrated that miR-21 inhibits chicken pre-adipocyte proliferation, at least in part, by targeting KLF5. |
| Databáze: | OpenAIRE |
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